Qualitatively and quantitatively investigating the metabolism of 20(S)-protopanaxadiol-type ginsenosides by gut microbiota of different species

doi:10.1002/bmc.5219

	Qualitatively and quantitatively investigating the metabolism of 20(S)-protopanaxadiol-type ginsenosides by gut microbiota of different species
	Zhang, Ying 1,2; Yao, Lingling 3,4,5; Tang, Chunping 4,5; Jiang, Jianlan 1; Ye, Yang 3,4,5; Liu, Jia 2
刊名	BIOMEDICAL CHROMATOGRAPHY
	2021-08-23
页码	8
关键词	gut microbiota LC-MS PPD-type ginsenosides species
ISSN号	0269-3879
DOI	10.1002/bmc.5219
通讯作者	Jiang, Jianlan(jljiang@tju.edu.cn) ; Ye, Yang(yye@simm.ac.cn) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要	Ginsenosides Rb1, Rb2, Rb3 and Rc, four major protopanaxadiol (PPD)-type ginsenosides, can be metabolized by gut microbiota. The composition of gut microbiota varies in different species. Existing publications have reported the metabolite fates of ginsenosides by gut microbiota from single species. However, their microbiota-related metabolic species differences have not been evaluated yet. In current study, in vitro anaerobic incubations of PPD-type ginsenosides with gut microbiota from humans, rabbits and rats were conducted. The metabolites of each ginsenoside were then identified by LC-MS. A total of 15 metabolites from the four ginsenosides were identified. The major metabolic pathways were stepwise removals of the C-20 and C-3 sugar moieties to obtain aglycone PPD. The results showed that the hydrolysis rate of C-20 terminal beta-D-glucopyranosyl was significantly higher than those of alpha-L-arabinopyranosyl, beta-D-xylopyranosyl and alpha-L-arabinofuranosyl in different species. The activity of beta-glucosidase, the metabolic rates of parent compounds and the formation rates of their metabolites were significantly higher in gut microbiota from rabbits than from humans and rats. Our research draws researchers' attention to the species differences of microbiota-related drug metabolism.
资助项目	Science and Technology Commission of Shanghai Municipality[20430780300] ; National Natural Science Foundation of China[81803610]
WOS关键词	HUMAN INTESTINAL MICROFLORA ; PANAX-QUINQUEFOLIUS ; GINSENG SAPONINS ; BIOTRANSFORMATION ; EXTRACT ; IDENTIFICATION ; PHARMACOLOGY ; BIOACTIVITY ; PROFILE ; GENE
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY
WOS记录号	WOS:000687349100001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297952]
专题	中国科学院上海药物研究所
通讯作者	Jiang, Jianlan; Ye, Yang; Liu, Jia
作者单位	1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300350, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ying,Yao, Lingling,Tang, Chunping,et al. Qualitatively and quantitatively investigating the metabolism of 20(S)-protopanaxadiol-type ginsenosides by gut microbiota of different species[J]. BIOMEDICAL CHROMATOGRAPHY,2021:8.
APA	Zhang, Ying,Yao, Lingling,Tang, Chunping,Jiang, Jianlan,Ye, Yang,&Liu, Jia.(2021).Qualitatively and quantitatively investigating the metabolism of 20(S)-protopanaxadiol-type ginsenosides by gut microbiota of different species.BIOMEDICAL CHROMATOGRAPHY,8.
MLA	Zhang, Ying,et al."Qualitatively and quantitatively investigating the metabolism of 20(S)-protopanaxadiol-type ginsenosides by gut microbiota of different species".BIOMEDICAL CHROMATOGRAPHY (2021):8.