HBO1 is a versatile histone acyltransferase critical for promoter histone acylations

doi:10.1093/nar/gkab607

	HBO1 is a versatile histone acyltransferase critical for promoter histone acylations
	Xiao, Yanhui 1,2,3,4; Li, Wenjing 5,6; Yang, Hui 7,8; Pan, Lulu 9; Zhang, Liwei 10; Lu, Lu 1,2; Chen, Jiwei 1,2; Wei, Wei 1,2; Ye, Jie 5,6; Li, Jiwen 1,2
刊名	NUCLEIC ACIDS RESEARCH
	2021-08-20
卷号	49 期号:14 页码:8037-8059
ISSN号	0305-1048
DOI	10.1093/nar/gkab607
通讯作者	Ding, Jianping(jpding@sibcb.ac.cn) ; Wong, Jiemin(jmweng@bio.ecnu.edu.cn)
英文摘要	Recent studies demonstrate that histones are subjected to a series of short-chain fatty acid modifications that is known as histone acylations. However, the enzymes responsible for histone acylations in vivo are not well characterized. Here, we report that HBO1 is a versatile histone acyltransferase that catalyzes not only histone acetylation but also propionylation, butyrylation and crotonylation both in vivo and in vitro and does so in a JADE or BRPF family scaffold protein-dependent manner. We show that the minimal HBO1/BRPF2 complex can accommodate acetyl-CoA, propionyl-CoA, butyryl-CoA and crotonyl-CoA. Comparison of CBP and HBO1 reveals that they catalyze histone acylations at overlapping as well as distinct sites, with HBO1 being the key enzyme for H3K14 acylations. Genome-wide chromatin immunoprecipitation assay demonstrates that HBO1 is highly enriched at and contributes to bulk histone acylations on the transcriptional start sites of active transcribed genes. HBO1 promoter intensity highly correlates with the level of promoter histone acylation, but has no significant correlation with level of transcription. We also show that HBO1 is associated with a subset of DNA replication origins. Collectively our study establishes HBO1 as a versatile histone acyltransferase that links histone acylations to promoter acylations and selection of DNA replication origins.
资助项目	Ministry of Science and Technology of China[2017YFA0504201] ; National Natural Science Foundation of China[31961133009] ; National Natural Science Foundation of China[31800622] ; National Natural Science Foundation of China[31801060] ; Shanghai Science and Technology Committee[20JC1411500] ; Chinese Academy of Sciences[XDB37030305]
WOS关键词	LYSINE 2-HYDROXYISOBUTYRYLATION ; GENE-EXPRESSION ; ACETYLATION ; CHROMATIN ; PROPIONYLATION ; ALIGNMENT ; COMPLEX
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000692599800023
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/297869]
专题	中国科学院上海药物研究所
通讯作者	Ding, Jianping; Wong, Jiemin
作者单位	1.East China Normal Univ, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, Shanghai 200241, Peoples R China 2.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China 3.East China Normal Univ, Joint Ctr Translat Med, Sch Life Sci, Shanghai 201499, Peoples R China 4.Fengxian Dist Cent Hosp, Shanghai 201499, Peoples R China 5.Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China 6.Univ Chinese Acad Sci, Shanghai 200031, Peoples R China 7.Tongji Univ, Translat Med Ctr Stem Cell Therapy, Shanghai 200092, Peoples R China 8.Tongji Univ, Inst Regenerat Med, Shanghai East Hosp, Sch Life Sci & Technol,Shanghai Key Lab Signaling, Shanghai 200092, Peoples R China 9.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 10.Chinese Acad Sci, CAS Ctr Excellence Biomacromol, Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Yanhui,Li, Wenjing,Yang, Hui,et al. HBO1 is a versatile histone acyltransferase critical for promoter histone acylations[J]. NUCLEIC ACIDS RESEARCH,2021,49(14):8037-8059.
APA	Xiao, Yanhui.,Li, Wenjing.,Yang, Hui.,Pan, Lulu.,Zhang, Liwei.,...&Wong, Jiemin.(2021).HBO1 is a versatile histone acyltransferase critical for promoter histone acylations.NUCLEIC ACIDS RESEARCH,49(14),8037-8059.
MLA	Xiao, Yanhui,et al."HBO1 is a versatile histone acyltransferase critical for promoter histone acylations".NUCLEIC ACIDS RESEARCH 49.14(2021):8037-8059.