Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts

doi:10.1021/acssynbio.0c00637

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 2012年后新成立研究组

	Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts
	Frangedakis, Eftychios ; Guzman-Chavez, Fernando ; Rebmann, Marius ; Markel, Kasey 2; Yu, Ying 3,7; Perraki, Artemis 8; Tse, Sze Wai ; Liu, Yang 1,9; Rever, Jenna ; Sauret-Gueto, Susanna
刊名	ACS SYNTHETIC BIOLOGY
	2021
卷号	10 期号:7 页码:1651-1666
关键词	Marchantia plant chloroplast plastome transcriptome gene assembly
ISSN号	2161-5063
DOI	10.1021/acssynbio.0c00637
英文摘要	Y Chloroplasts are attractive platforms for synthetic biology applications since they are capable of driving very high levels of transgene expression, if mRNA production and stability are properly regulated. However, plastid transformation is a slow process and currently limited to a few plant species. The liverwort Marchantia polymorpha is a simple model plant that allows rapid transformation studies; however, its potential for protein hyper-expression has not been fully exploited. This is partially due to the fact that chloroplast post-transcriptional regulation is poorly characterized in this plant. We have mapped patterns of transcription in Marchantia chloroplasts. Furthermore, we have obtained and compared sequences from 51 bryophyte species and identified putative sites for pentatricopeptide repeat protein binding that are thought to play important roles in mRNA stabilization. Candidate binding sites were tested for their ability to confer high levels of reporter gene expression in Marchantia chloroplasts, and levels of protein production and effects on growth were measured in homoplastic transformed plants. We have produced novel DNA tools for protein hyperexpression in this facile plant system that is a test-bed for chloroplast engineering.
学科主题	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000674933400009
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/12328]
专题	西双版纳热带植物园_2012年后新成立研究组
作者单位	1.Fairy Lake Bot Garden, Shenzhen 518004, Guangdong, Peoples R China 2.Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England 3.Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA 4.Chinese Acad Sci, Ctr Integrat Conservat, Xishuangbanna Trop Bot Garden, Menglun 666303, Yunnan, Peoples R China 5.Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA 6.Univ Cambridge, Crop Sci Ctr, 93 Lawrence Weaver Rd, Cambridge CB3 0LE, England 7.Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 311121, Peoples R China 8.Huangshan Univ, Coll Life & Environm Sci, Huangshan 245041, Peoples R China 9.Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion 70013, Crete, Greece 10.Chinese Acad Sci, Shenzhen 518004, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Frangedakis, Eftychios,Guzman-Chavez, Fernando,Rebmann, Marius,et al. Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts[J]. ACS SYNTHETIC BIOLOGY,2021,10(7):1651-1666.
APA	Frangedakis, Eftychios.,Guzman-Chavez, Fernando.,Rebmann, Marius.,Markel, Kasey.,Yu, Ying.,...&Haseloff, Jim.(2021).Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts.ACS SYNTHETIC BIOLOGY,10(7),1651-1666.
MLA	Frangedakis, Eftychios,et al."Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts".ACS SYNTHETIC BIOLOGY 10.7(2021):1651-1666.