Preferential flow has always been hotspot of research regarding soil water flow, biological activity, and carbon and nitrogen dynamics. However, the mechanism of water flow exchange between two adjacent zones (with and without root system) and the pattern of soil water supply for rubber are still unclear. In the present study, we considered two plots experiencing similar farming history: in the first plot we measured soil physical properties and soil volumetric water content (VWC) during rainfall, and the second plot was used to visualise water flow path and to measure root biomass. Besides, a model was developed on preferential flow domain (PFD, matching the root zone) and on non-preferential flow domain (NPFD, matching the remote root zone) to compute differences in soil properties between the PFD and NPFD. The results revealed that the dominant flow type in the PFD was preferential flow and the one in the NPFD was capillary flow. Dye stained area and wetting front rate negatively correlated with bulk density, while they positively correlated to non-capillary porosity and root biomass. Accordingly, PFD showed a quick response to rainfall. Indeed, during the rain, a lateral flow (driven by water gravity and pressure head gradient) predominantly carried water (0.95, 0.27, and 0.44 cm(3) cm(-3) for various rainfall events 1, 2, and 3, respectively) from PFD to NPFD. During the soil drainage stage, the lateral flow direction changed, and water (about 0.63, 0.30, and 0.39 cm(3) cm(-3) for rainfall events 1, 2, and 3, respectively) flowed from NPFD to PFD. As a result, PFD presented low storage and high flow characteristics compared with NPFD, suggesting this complementary relationship for water interaction between the two domains could be beneficial for rubber plants growth and development.