Exposure to environmentally relevant concentrations of deltamethrin renders the Chinese rare minnow (Gobiocypris rarus) vulnerable to Pseudomonas fluorescens infection

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Exposure to environmentally relevant concentrations of deltamethrin renders the Chinese rare minnow (Gobiocypris rarus) vulnerable to Pseudomonas fluorescens infection
	Zhang, Le; Hong, Xiangsheng; Zhao, Xu; Yan, Saihong; Ma, Xufa; Zha, Jinmiao
刊名	SCIENCE OF THE TOTAL ENVIRONMENT
	2020-05-01
卷号	715 页码:1-11
关键词	Chinese rare minnow Deltamethrin Immunotoxicity Immune responses Pseudomonas fluorescens
ISSN号	0048-9697
英文摘要	In this study, to assess the immunotoxicity of deltamethrin on fish, adult Chinese rare minnows (Gobiocypris rarus) were exposed to 0.1,0.3, and 1 mu g/L deltamethrin for 28 d. Many immunological parameters and histopathological alterations were determined. The results showed that lymphocyte number was markedly decreased at 0.3 and 1 mu g/L treatments, whereas the neutrophil number was strongly increased at 1 mu g/L treatments (p < 0.05). Furthermore, lysozyme (LYS), immunoglobulin M (IgM), and complement component 3 (C3) levels at 0.3 and 1 mu g/L treatments were markedly reduced, whereas alkaline phosphatase (ALP) activity were marked increased at 1 mu g/L treatments (p < 0.05). The transcripts of almost all TLR (Toll-like receptor) signaling pathwayrelated genes were up-regulated. Histological lesions in the livers, intestines, and gills were observed at all treatments. Then, all remaining fish from controls and deltamethrin-exposed groups were injected with Pseudomonas fluorescens (P. fluorescens) for 48 h. At 24 and 48 h post-injection with P. fluorescens (hpi), the lymphocyte numbers were strongly reduced at 0.3 and 1 mu g/L deltamethrin-exposed groups, whereas LYS and C3 levels were strongly reduced at 0.3 and 1 mu g/L deltamethrin-exposed groups (p < 0.05). Obvious reduces in 1gM levels were also detected at 0.3 and 1 mu g/Ldeltamethrin-exposed groups at 48 hpi (p <- 0.05). The transcripts of almost all TLR signaling pathway-related genes were significantly down-regulated, whereas the levels of related microRNAs (miRNAs) were markedly increased at all deltamethrin-exposed groups at 24 and 48 hpi. Moreover, the bacterial load in the liver and the mortality of fish were significantly increased at 1 mu g/Ldeltamethrin-exposed groups at 24 and 48 hpi (p < 0.05). Furthermore, obvious histological damage in the livers, intestines, and gills were observed at all deltamethrin-exposed fish at 48 hpi. Overall, our results demonstrated that environmentally relevant concentration deltamethrin suppressed immunity and rendered the fish vulnerable to P. fluorescens infection, subsequently inducing mortality. (C) 2020 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45295]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Zhang, Le,Hong, Xiangsheng,Zhao, Xu,et al. Exposure to environmentally relevant concentrations of deltamethrin renders the Chinese rare minnow (Gobiocypris rarus) vulnerable to Pseudomonas fluorescens infection[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,715:1-11.
APA	Zhang, Le,Hong, Xiangsheng,Zhao, Xu,Yan, Saihong,Ma, Xufa,&Zha, Jinmiao.(2020).Exposure to environmentally relevant concentrations of deltamethrin renders the Chinese rare minnow (Gobiocypris rarus) vulnerable to Pseudomonas fluorescens infection.SCIENCE OF THE TOTAL ENVIRONMENT,715,1-11.
MLA	Zhang, Le,et al."Exposure to environmentally relevant concentrations of deltamethrin renders the Chinese rare minnow (Gobiocypris rarus) vulnerable to Pseudomonas fluorescens infection".SCIENCE OF THE TOTAL ENVIRONMENT 715(2020):1-11.