Dissimilatory nitrate reduction to ammonium (DNRA) in traditional municipal wastewater treatment plants in China: Widespread but low contribution

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院饮用水科学与技术重点实验室

	Dissimilatory nitrate reduction to ammonium (DNRA) in traditional municipal wastewater treatment plants in China: Widespread but low contribution
	Wang, Shanyun; Liu, Chunlei; Wang, Xiaoxia; Yuan, Dongdan; Zhu, Guibing
刊名	WATER RESEARCH
	2020-07-15
卷号	179 页码:1-9
关键词	Dissimilatory nitrate reduction to ammonium (DNRA) Denitrification Anammox Nitrospira NO3- Reduction Municipal wastewater treatment plants (WWTPs)
ISSN号	0043-1354
英文摘要	Recent reports on the occurrence and contribution of dissimilatory nitrate reduction to ammonium (DNRA) in marine, inland water, and soil systems have greatly improved our understanding of the global nitrogen (N) cycle. This also promoted the investigation of the role and ecological features of DNRA in anthropogenic ecosystems. However, so far, the use of DNRA in municipal wastewater treatment plants (WWTPs), which are one of the most common and largest biotechnologically artificial water ecosystems, has not been investigated. Accordingly, this study focused on the abundance, activity, community structure, and diversity of DNRA bacteria in full-scale WWTPs. DNRA bacteria were detected in all treatment units in six tested municipal WWTPs, even in aerobic zones (dissolved oxygen > 2 mg L-1). Although the relative abundance of DNRA bacteria (0.2-4.0%) was less than that of denitrifying bacteria (0.7-10.1%) among all investigated samples, the abundance of DNRA bacteria still reaches 10(9) gene copies g(-1). However, N-15-isotope tracing indicated that the potential DNRA rates were significantly lower (0.4-2.1 nmol N g(-1) h(-1)) than those of denitrification (9.5-15.7 nmol N g(-1) h(-1)), but higher than anammox rate (0.3-1.3 nmol N g(-1) h(-1)). The DNRA bacterial community structure was primarily affected by temperature gradient despite the treatment process. High-throughput sequencing analysis targeting the DNRA nrfA gene showed that Nitrospira accounted for the largest proportion of nrfA genes among all samples (6.2-36.3%), followed by Brocadia (5.9-22.1%). Network analysis further indicated that Nitrospira played an important role in both the DNRA bacterial community and entire bacterial community in municipal WWTPs. These results suggest that the ecological habitats of DNRA bacteria in anthropogenic ecosystems were far more abundant than previously assumed. However, the contribution to N transformation by the widespread DNRA was not significant in traditional municipal WWTPs. (c) 2020 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45266]
专题	生态环境研究中心_中国科学院饮用水科学与技术重点实验室
推荐引用方式 GB/T 7714	Wang, Shanyun,Liu, Chunlei,Wang, Xiaoxia,et al. Dissimilatory nitrate reduction to ammonium (DNRA) in traditional municipal wastewater treatment plants in China: Widespread but low contribution[J]. WATER RESEARCH,2020,179:1-9.
APA	Wang, Shanyun,Liu, Chunlei,Wang, Xiaoxia,Yuan, Dongdan,&Zhu, Guibing.(2020).Dissimilatory nitrate reduction to ammonium (DNRA) in traditional municipal wastewater treatment plants in China: Widespread but low contribution.WATER RESEARCH,179,1-9.
MLA	Wang, Shanyun,et al."Dissimilatory nitrate reduction to ammonium (DNRA) in traditional municipal wastewater treatment plants in China: Widespread but low contribution".WATER RESEARCH 179(2020):1-9.