Variety features differentiate microbiota in the grape leaves

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Variety features differentiate microbiota in the grape leaves
	Zhang, Shiwei; Wang, Yuan; Chen, Xi; Cui, Bingjian; Bai, Zhihui; Zhuang, Guoqiang
刊名	CANADIAN JOURNAL OF MICROBIOLOGY
	2020-11
卷号	66 期号:11 页码:653-663
关键词	grape leaves bacterial 16S rRNA gene fungal internal transcribed spacer (ITS) high-throughput sequencing
ISSN号	0008-4166
英文摘要	The dependence of plant health and crop quality on the epiphytic microbial community has been extensively addressed, but little is known about plant-associated microbial communities under natural conditions. In this study, the bacterial and fungal communities on grape leaves were analyzed by 16S rRNA gene and internal transcribed spacer high-throughput sequencing, respectively. The results showed differences in the composition of the microbial communities on leaf samples of nine wine grape varieties. The most abundant bacterial genus was Pseudomonas, and the top three varieties with Pseudomonas were Zinfandel (22.6%), Syrah (21.6%), and Merlot (13.5%). The most abundant fungal genus was Alternaria, and the cultivar with the lowest abundance of Alternaria was Zinfandel (33.6%), indicating that these communities had different habitat preferences. The linear discriminant analysis effect size of all species showed that the bacteria Enterococcus, Massilia, and Kocuria were significantly enriched on the leaves of Merlot, Syrah, Cabernet Sauvignon, respectively; Pseudomonadales and Pantoea on Zinfandel; and Bacillus, Turicibacter, and Romboutsia on Pinot Noir. Similarly, the fungi Cladosporium, Phoma, and Sporormiella were significantly enriched on Zinfandel, Lon, and Gem, respectively. Both Bray-Curtis and unweighted UniFrac revealed that bacteria and fungi have a significant impact (P < 0.01), and the results further proved that variety is the most important factor affecting the microbial community. The findings indicate that some beneficial or harmful microorganisms existing on the wine grape leaves might affect the health of the grape plants and the wine-making process.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45222]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Zhang, Shiwei,Wang, Yuan,Chen, Xi,et al. Variety features differentiate microbiota in the grape leaves[J]. CANADIAN JOURNAL OF MICROBIOLOGY,2020,66(11):653-663.
APA	Zhang, Shiwei,Wang, Yuan,Chen, Xi,Cui, Bingjian,Bai, Zhihui,&Zhuang, Guoqiang.(2020).Variety features differentiate microbiota in the grape leaves.CANADIAN JOURNAL OF MICROBIOLOGY,66(11),653-663.
MLA	Zhang, Shiwei,et al."Variety features differentiate microbiota in the grape leaves".CANADIAN JOURNAL OF MICROBIOLOGY 66.11(2020):653-663.