Application of biofertilizer containing Bacillus subtilis reduced the nitrogen loss in agricultural soil

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 中国科学院环境生物技术重点实验室

	Application of biofertilizer containing Bacillus subtilis reduced the nitrogen loss in agricultural soil
	Sun, Bo; Gu, Likun; Bao, Lijun; Zhang, Shiwei; Wei, Yingxue; Bai, Zhihui; Zhuang, Guoqiang; Zhuang, Xuliang
刊名	SOIL BIOLOGY & BIOCHEMISTRY
	2020-09
卷号	148 页码:1-12
关键词	Biofertilizer Bacillus subtilis Non-point pollution Nitrogen loss Functional genes Microbial community
ISSN号	0038-0717
英文摘要	Nitrogen losses caused by excess fertilizer application in agriculture are one of the main sources of non-point pollution. Biofertilizer has been considered as an effective alternative to synthetic nitrogen fertilizer, but the effectiveness and mechanism for controlling non-point pollution remains unclear. In this study, the effect of biofertilizer containing Bacillus subtilis on nitrogen loss in agricultural soil was investigated. Compared with the application of urea alone, the strategy of substituting 50% urea with biofertilizer reduced the nitrogen loss from farmland soil by 54%. Moreover, this strategy also increased nitrogen use efficiency by 11.2% and achieved a 5.0% increase in crop yield. Application of biofertilizer decreased the abundance of bacterial amoA gene and increased the abundance of narG, nirS, nirK, and nosZ genes in soil. This implied a decrease in nitrification and an increase in denitrification. Thus, it reduced the accumulation of NO3--N in soil and greatly reduced nitrogen runoff and leaching loss. In addition, biofertilizer decreased the abundance of nitrogen-fixing gene nifH by up to 2 times. Application of biofertilizer also increased the abundance of Bacteroidetes and Chloroflexi, which play important roles in degradation of soil organic matter. In conclusion, the biofertilizer regulated the microbial nitrogen transformation process in soil and reduced nitrogen loss from agroecosystems.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/45134]
专题	生态环境研究中心_中国科学院环境生物技术重点实验室
推荐引用方式 GB/T 7714	Sun, Bo,Gu, Likun,Bao, Lijun,et al. Application of biofertilizer containing Bacillus subtilis reduced the nitrogen loss in agricultural soil[J]. SOIL BIOLOGY & BIOCHEMISTRY,2020,148:1-12.
APA	Sun, Bo.,Gu, Likun.,Bao, Lijun.,Zhang, Shiwei.,Wei, Yingxue.,...&Zhuang, Xuliang.(2020).Application of biofertilizer containing Bacillus subtilis reduced the nitrogen loss in agricultural soil.SOIL BIOLOGY & BIOCHEMISTRY,148,1-12.
MLA	Sun, Bo,et al."Application of biofertilizer containing Bacillus subtilis reduced the nitrogen loss in agricultural soil".SOIL BIOLOGY & BIOCHEMISTRY 148(2020):1-12.