Temporal Dynamics of Antibiotic Resistome in the Plastisphere during Microbial Colonization

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Temporal Dynamics of Antibiotic Resistome in the Plastisphere during Microbial Colonization
	Yang, Kai; Chen, Qing-Lin; Chen, Mo-Lian; Li, Hong-Zhe; Liao, Hu; Pu, Qiang; Zhu, Yong-Guan; Cui, Li
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2020-09-15
卷号	54 期号:18 页码:11322-11332
ISSN号	0013-936X
英文摘要	The increasing and simultaneous pollution of plastic debris and antibiotic resistance in aquatic environments makes plastisphere a great health concern. However, the development process of antibiotic resistome in the plastisphere is largely unknown, impeding risk assessment associated with plastics. Here, we profiled the temporal dynamics of antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and microbial composition in the plastisphere from initial microbial colonization to biofilm formation in urban water. A total of 82 ARGs, 12 MGEs, and 63 bacterial pathogens were detected in the plastisphere and categorized as the pioneering, intermediate, and persistent ones. The high number of five MGEs and six ARGs persistently detected in the whole microbial colonization process was regarded as a major concern because of their potential role in disseminating antibiotic resistance. In addition to genomic analysis, D2O-labeled single-cell Raman spectroscopy was employed to interrogate the ecophysiology of plastisphere in a culture-independent way and demonstrated that the plastisphere was inherently more tolerant to antibiotics than bacterioplankton. Finally, by combining persistent MGEs, intensified colonization of pathogenic bacteria, increased tolerance to antibiotic, and potential trophic transfer into a holistic risk analysis, the plastisphere was indicated to constitute a hot spot to acquire and spread antibiotic resistance and impose a long-term risk to ecosystems and human health. These findings provide important insights into the antibiotic resistome and ecological risk of the plastisphere and highlight the necessity for comprehensive surveillance of plastisphere.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44477]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	Yang, Kai,Chen, Qing-Lin,Chen, Mo-Lian,et al. Temporal Dynamics of Antibiotic Resistome in the Plastisphere during Microbial Colonization[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2020,54(18):11322-11332.
APA	Yang, Kai.,Chen, Qing-Lin.,Chen, Mo-Lian.,Li, Hong-Zhe.,Liao, Hu.,...&Cui, Li.(2020).Temporal Dynamics of Antibiotic Resistome in the Plastisphere during Microbial Colonization.ENVIRONMENTAL SCIENCE & TECHNOLOGY,54(18),11322-11332.
MLA	Yang, Kai,et al."Temporal Dynamics of Antibiotic Resistome in the Plastisphere during Microbial Colonization".ENVIRONMENTAL SCIENCE & TECHNOLOGY 54.18(2020):11322-11332.