Microbiome and antibiotic resistome in household dust from Beijing, China

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Microbiome and antibiotic resistome in household dust from Beijing, China
	Ding, Long-Jun; Zhou, Xin-Yuan; Zhu, Yong-Guan
刊名	ENVIRONMENT INTERNATIONAL
	2020-06
卷号	139 页码:1-10
关键词	Microbial diversity Geographical distribution Bacteria Fungi Antibiotic resistant genes Antibiotic Household
ISSN号	0160-4120
英文摘要	We spend ever-increasing time indoors along with urbanization; however, the geographical distribution patterns of microbiome and antibiotic resistome, and their driving forces in household environment remains poorly characterized. Here, we surveyed the bacterial and fungal communities, and the resistome in settled dust gathered from 82 homes located across Beijing, China, employing Illumina sequencing and high-throughput quantitative PCR techniques. There was no clear geographical distribution pattern in dust-related bacterial communities although a slight but significant (P < 0.05) distance-decay relationship occurred in its community similarity; by contrast, a relatively distinct geographical clustering and a stronger distance-decay relationship were observed in fungal communities at the local scale. The cross-domain (bacteria versus fungi) relationships in the microbiome of the dust samples were mostly observed as robust co-occurrence correlations. The bacterial communities were dominated by Proteobacteria and Actinobacteria phyla, with human skin, soil and plants being potential major sources. The fungal communities largely comprised potential allergens (a median 61% of the fungal sequences), with Alternaria genus within Ascomycota phylum being the most predominant taxa. The profile of dust-related bacterial communities was mainly affected by housing factors related to occupants and houseplants, while that of fungal communities was determined by georeferenced environmental factors, particularly vascular plant diversity. Additionally, a great diversity (1.96 on average for Shannon index) and normalized abundance (2.22 copies per bacterial cell on average) of antibiotic resistance genes were detected across the dust samples, with the dominance of genes resistant to vancomycin and Macrolide-Lincosamide-Streptogramin B. The resistome profile exhibited no distinct geographical pattern, and was primarily driven by certain bacterial phyla and occupancy-related factors. Overall, we underline the significance of anthropogenic impacts and house location in structuring bacterial and fungal communities inside homes, respectively, and suggest that household dust is an overlooked reservoir for antibiotic resistance.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44382]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	Ding, Long-Jun,Zhou, Xin-Yuan,Zhu, Yong-Guan. Microbiome and antibiotic resistome in household dust from Beijing, China[J]. ENVIRONMENT INTERNATIONAL,2020,139:1-10.
APA	Ding, Long-Jun,Zhou, Xin-Yuan,&Zhu, Yong-Guan.(2020).Microbiome and antibiotic resistome in household dust from Beijing, China.ENVIRONMENT INTERNATIONAL,139,1-10.
MLA	Ding, Long-Jun,et al."Microbiome and antibiotic resistome in household dust from Beijing, China".ENVIRONMENT INTERNATIONAL 139(2020):1-10.