Prevalence and characterization of oxazolidinone and phenicol cross-resistance gene optr A in enterococci obtained from anaerobic digestion systems treating swine manure

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境水质学国家重点实验室

	Prevalence and characterization of oxazolidinone and phenicol cross-resistance gene optr A in enterococci obtained from anaerobic digestion systems treating swine manure
	Yang, Xiao-Xiao; Tian, Tian-Tian; Qiao, Wei; Tian, Zhe; Yang, Min; Zhang, Yu; Li, Jiu-Yi
刊名	ENVIRONMENTAL POLLUTION
	2020-12
卷号	267 页码:1-4
关键词	Antibiotic resistance genes Florfenicol Linezolid Enterococci Anaerobic digestion Swine waste
ISSN号	0269-7491
英文摘要	The use of the phenicol antibiotic florfenicol in livestock can select for the optrA gene, which also confers resistance to the critically important oxazolidinone antibiotic linezolid. However, the occurrence and dissemination of florfenicol and linezolid cross-resistance genes in anaerobic treatment systems for livestock waste are unknown. Herein, the phenotypes and genotypes (optrA, fexA, fexB, and cfr) of florfenicol and linezolid cross-resistance were investigated in 339 enterococci strains isolated from laband full-scale mesophilic anaerobic digestion systems treating swine waste. It was found that optrA, fexA, and fexB were frequently detected in isolated enterococci in both systems by PCR screening, whereas cfr was not detected. The most abundant gene was optrA, which was detected in 73.5% (n = 50) and 38.9% (n = 23) of enterococci isolates in the full-scale influent and effluent, respectively. Most strains carried more than two resistance genes, and the average percentage of co-occurrence of optrA/fexA was 16.6%. Based on minimum inhibitory concentrations of the enterococci strain phenotypes, 85.7%, 77.5%, and 77.5% of strains in influent were resistant to chloramphenicol, florfenicol, and linezolid, respectively, while 56.3%, 65.2%, and 13% in the effluent isolates were found, respectively, which was consistent with the genotype results. The phenotypes and genotypes of florfenicol and linezolid resistance were relative stable in the enterococci isolated from the influent and effluent in lab-scale anaerobic digestion system. The findings signify the enterococci isolates harboring the optrA gene remained in effluents of both full and lab-scale swine waste anaerobic digestion system; hence, effective management strategies should be implemented to prevent the discharge of antibiotic resistance from the livestock waste treatment systems. (c) 2020 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44982]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Yang, Xiao-Xiao,Tian, Tian-Tian,Qiao, Wei,et al. Prevalence and characterization of oxazolidinone and phenicol cross-resistance gene optr A in enterococci obtained from anaerobic digestion systems treating swine manure[J]. ENVIRONMENTAL POLLUTION,2020,267:1-4.
APA	Yang, Xiao-Xiao.,Tian, Tian-Tian.,Qiao, Wei.,Tian, Zhe.,Yang, Min.,...&Li, Jiu-Yi.(2020).Prevalence and characterization of oxazolidinone and phenicol cross-resistance gene optr A in enterococci obtained from anaerobic digestion systems treating swine manure.ENVIRONMENTAL POLLUTION,267,1-4.
MLA	Yang, Xiao-Xiao,et al."Prevalence and characterization of oxazolidinone and phenicol cross-resistance gene optr A in enterococci obtained from anaerobic digestion systems treating swine manure".ENVIRONMENTAL POLLUTION 267(2020):1-4.