Microbial community functional structure in an aerobic biofilm reactor: Impact of streptomycin and recovery

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	Microbial community functional structure in an aerobic biofilm reactor: Impact of streptomycin and recovery
	Luan, Xiao; Zhang, Hong; Tian, Zhe; Yang, Min; Wen, Xianghua; Zhang, Yu
刊名	CHEMOSPHERE
	2020-09
卷号	255 页码:1-15
关键词	Wastewater treatment systems Antibiotic exposure Microbial community Functional genes Antibiotic resistance genes
ISSN号	0045-6535
英文摘要	Antibiotics can affect microbial community structure and promote antibiotic resistance. However, the course of microbial community recovery in wastewater treatment systems after antibiotic disturbance remains unclear. Herein, multiple molecular biology tools, including 16S amplicon sequencing, GeoChip 5.0, quantitative polymerase chain reaction (qPCR), and metagenomic sequencing, were used to investigate the year-long (352 d) recovery of the microbial community functional structure in an aerobic biofilm reactor. Nitrification was completely inhibited under 50 mg/L of streptomycin spiking (STM_50) due to the significant reduction of ammonia-oxidizing bacteria, but recovered to original pre-disturbance levels after streptomycin removal, indicating the high resilience of ammonia-oxidizing bacteria. Bacterial community richness and diversity decreased significantly under STM_50 (p < 0.05), but recovered to levels similar to those observed before disturbance after 352 d. In contrast, bacterial composition did not recover to the original structure. The carbon degradation and nitrogen cycling functional community significantly changed after recovery compared to that observed pre-disturbance (p < 0.05), thus indicating functional redundancy. Additionally, levels of aminoglycoside and total antibiotic resistance genes under STM_50 (relative abundance, 0.33 and 0.80, respectively) and after one year of recovery (0.12 and 0.29, respectively) were higher than the levels detected pre-disturbance (0.04 and 0.24, respectively). This study provides an overall depiction of the recovery of the microbial community functional structure after antibiotic exposure. Our findings give notice that recovery caused by antibiotic disturbance in the water environment should be taken more seriously, and that engineering control strategies should be implemented to prevent the antibiotic pollution of wastewater. (C) 2020 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44962]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Luan, Xiao,Zhang, Hong,Tian, Zhe,et al. Microbial community functional structure in an aerobic biofilm reactor: Impact of streptomycin and recovery[J]. CHEMOSPHERE,2020,255:1-15.
APA	Luan, Xiao,Zhang, Hong,Tian, Zhe,Yang, Min,Wen, Xianghua,&Zhang, Yu.(2020).Microbial community functional structure in an aerobic biofilm reactor: Impact of streptomycin and recovery.CHEMOSPHERE,255,1-15.
MLA	Luan, Xiao,et al."Microbial community functional structure in an aerobic biofilm reactor: Impact of streptomycin and recovery".CHEMOSPHERE 255(2020):1-15.