Binding and activation of estrogen related receptor gamma as possible molecular initiating events of hydroxylated benzophenones endocrine disruption toxicity

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Binding and activation of estrogen related receptor gamma as possible molecular initiating events of hydroxylated benzophenones endocrine disruption toxicity
	Zheng, Xiyue; Ren, Xiao-Min; Zhao, Lixia; Guo, Liang-Hong
刊名	ENVIRONMENTAL POLLUTION
	2020-08
卷号	263 页码:1-12
关键词	UV-Filters Hydroxylated benzophenones Estrogen related receptor gamma Transcriptional activity Endocrine disruption
ISSN号	0269-7491
英文摘要	As a class of widely used ultraviolet filters, hydroxylated benzophenones (BPs) have been detected in various environmental and human samples, raising concerns about their ecological and health effects. BPs were found to impair reproductive functions, and their disruption effects on estrogen receptor, G protein-coupled estrogen receptor, and androgen receptor were investigated as possible mechanisms of action. In the present study, we investigated the binding and activation of estrogen related receptor gamma (ERR gamma) as an alternative mechanism of BPs reproductive toxicity. In competitive fluorescence binding assays, all of the 8 tested BPs were found to bind to the ligand-binding domain of ERR gamma but with varying binding affinity. Molecular dock analysis indicated that binding of BPs to ERR gamma in the agonistic conformation is preferred. In luciferase reporter gene assays in HepG2 cells, all the 8 BPs activated the transcription of ERR gamma in a concentration-dependent manner in the range of 3-100 mu M,consistent with the docking result. Hazard quotient calculation revealed a high risk on the BP-type personal care products users. The combined in vitro and in silico results suggest that binding and activation of ERR gamma might be the molecular initiating events of an alternative mechanism of BPs endocrine disruption toxicity. (C) 2020 Published by Elsevier Ltd.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44615]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Zheng, Xiyue,Ren, Xiao-Min,Zhao, Lixia,et al. Binding and activation of estrogen related receptor gamma as possible molecular initiating events of hydroxylated benzophenones endocrine disruption toxicity[J]. ENVIRONMENTAL POLLUTION,2020,263:1-12.
APA	Zheng, Xiyue,Ren, Xiao-Min,Zhao, Lixia,&Guo, Liang-Hong.(2020).Binding and activation of estrogen related receptor gamma as possible molecular initiating events of hydroxylated benzophenones endocrine disruption toxicity.ENVIRONMENTAL POLLUTION,263,1-12.
MLA	Zheng, Xiyue,et al."Binding and activation of estrogen related receptor gamma as possible molecular initiating events of hydroxylated benzophenones endocrine disruption toxicity".ENVIRONMENTAL POLLUTION 263(2020):1-12.