A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer
	Liu, Liying; Zhao, Qiang
刊名	JOURNAL OF ENVIRONMENTAL SCIENCES
	2020-11
卷号	97 页码:19-24
关键词	Bisphenol A Aptamer Fluorescence anisotropy Fluorescence polarization Microscale thermophoresis Binding affinity
ISSN号	1001-0742
英文摘要	Bisphenol A (BPA) is one of the environmental endocrine disruptors (EDCs), and BPA contamination in environment can cause high risks to human health. Rapid determination of BPA on sites is in high demand in environmental analysis. Taking advantage of aptamers as affinity ligands and fluorescence anisotropy (FA) analysis, we developed a simple and rapid FA assay for BPA by employing a single tetramethylrhodamine (TMR) labeled short 35-mer DNA aptamer against BPA. The assay is based on the BPA-binding induced conformation change of TMR-labeled aptamer and alteration of interaction between TMR and guanine bases, resulting in change of FA signals. We screened the FA change of aptamer probes having TMR label on a specific site of the aptamer upon BPA addition. The aptamer with a TMR label on the 22nd T base showed large FA-decreasing response to BPA and maintained good binding affinity to BPA. By using this TMR-labeled aptamer, we achieved FA detection of BPA with a detection limit of 0.5 Amol/L. under the optimized conditions. This assay was selective towards BPA and enabled the detection of BPA spiked in tap water sample, showing the potential applications on water samples. 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44587]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Liying,Zhao, Qiang. A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2020,97:19-24.
APA	Liu, Liying,&Zhao, Qiang.(2020).A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer.JOURNAL OF ENVIRONMENTAL SCIENCES,97,19-24.
MLA	Liu, Liying,et al."A simple fluorescence anisotropy assay for detection of bisphenol A using fluorescently labeled aptamer".JOURNAL OF ENVIRONMENTAL SCIENCES 97(2020):19-24.