Separation and identification of flavonoids from complex samples using off-line two-dimensional liquid chromatography tandem mass spectrometry

	Separation and identification of flavonoids from complex samples using off-line two-dimensional liquid chromatography tandem mass spectrometry
	Zeng, Jing 1,2; Zhang, Xiuli 1; Guo, Zhimou 1; Feng, Jiatao 1; Zeng, Jianguo 2; Xue, Xingya 1; Liang, Xinmiao 1
刊名	journal of chromatography a
	2012-01-13
卷号	1220 页码:50-56
关键词	Off-line two-dimensional liquid chromatography Identification Flavonoids Library searching Traditional Chinese Medicines
ISSN号	0021-9673
通讯作者	薛兴亚 ; 梁鑫淼
产权排序	1,1
英文摘要	in this study, a lc-based identification strategy was proposed and off-line two-dimensional liquid chromatography was developed for the separation and identification of flavonoids from complex samples. the highly orthogonal separation system was composed of a cd column, an oeg column and an xterra c-18 column. the cd column was employed for the first dimensional separation and the oeg column and xterra c-18 column were used for the second dimensional separation in parallel. a mixture of six traditional chinese medicines was used as a complex sample in testing this method. unknown peaks were identified by matching their retention times and accurate mass molecular weights with those of references. the identification efficacy of parallel unidimensional liquid chromatography and off-line two-dimensional liquid chromatography was compared, and the latter was demonstrated to be more efficient for the identification of target compounds-especially co-eluted compounds or minor compounds from complex samples. (c) 2011 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	reversed-phase ; oligo(ethylene glycol) ; stationary-phase ; selectivity ; toxicology ; systems ; silica ; click
收录类别	SCI
语种	英语
WOS记录号	WOS:000299586000008
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118414]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning Provin, Peoples R China 2.Hunan Agr Univ, Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Jing,Zhang, Xiuli,Guo, Zhimou,et al. Separation and identification of flavonoids from complex samples using off-line two-dimensional liquid chromatography tandem mass spectrometry[J]. journal of chromatography a,2012,1220:50-56.
APA	Zeng, Jing.,Zhang, Xiuli.,Guo, Zhimou.,Feng, Jiatao.,Zeng, Jianguo.,...&Liang, Xinmiao.(2012).Separation and identification of flavonoids from complex samples using off-line two-dimensional liquid chromatography tandem mass spectrometry.journal of chromatography a,1220,50-56.
MLA	Zeng, Jing,et al."Separation and identification of flavonoids from complex samples using off-line two-dimensional liquid chromatography tandem mass spectrometry".journal of chromatography a 1220(2012):50-56.