Metabolomics Study of Stepwise Hepatocarcinogenesis From the Model Rats to Patients: Potential Biomarkers Effective for Small Hepatocellular Carcinoma Diagnosis

	Metabolomics Study of Stepwise Hepatocarcinogenesis From the Model Rats to Patients: Potential Biomarkers Effective for Small Hepatocellular Carcinoma Diagnosis
	Tan, Yexiong 2; Yin, Peiyuan 1; Tang, Liang 2; Xing, Wenbin 3; Huang, Qiang 1; Cao, Dan 2; Zhao, Xinjie 1; Wang, Wenzhao 1; Lu, Xin 1; Xu, Zhiliang 1
刊名	molecular & cellular proteomics
	2012-02-01
卷号	11 期号:2 页码:106941
通讯作者	hongyangwang ; 许国旺
产权排序	2,2
英文摘要	the aim of this study is to find the potential biomarkers from the rat hepatocellular carcinoma (hcc) disease model by using a non-target metabolomics method, and test their usefulness in early human hcc diagnosis. the serum metabolic profiling of the diethylnitrosamine-induced rat hcc model, which presents a stepwise histopathological progression that is similar to human hcc, was performed using liquid chromatography-mass spectrometry. multivariate data analysis methods were utilized to identify the potential biomarkers. three metabolites, taurocholic acid, lysophosphoethanolamine 16: 0, and lysophosphatidylcholine 22:5, were defined as "marker metabolites," which can be used to distinguish the different stages of chemical hepatocarcinogenesis. these metabolites represented the abnormal metabolism during the progress of hepatocarcinogenesis, which could also be found in patients. to test their diagnosis potential 412 sera from 262 patients with hcc, 76 patients with cirrhosis and 74 patients with chronic hepatitis b were collected and studied, it was found that 3 marker metabolites were effective for the discrimination of small liver tumor (solitary nodules of less than 2 cm in diameter) patients, achieved a sensitivity of 80.5% and a specificity of 80.1%, which is better than those of alpha-fetoprotein (53 and 64%, respectively). moreover, they were also effective for the discrimination of all hccs and chronic liver disease patients, which could achieve a sensitivity of 87.5% and a specificity of 72.3%, better than those of alpha-fetoprotein (61.2 and 64%). these results indicate metabolomics method has the potential of finding biomarkers for the early diagnosis of hcc. molecular & cellular proteomics 11: 10.1074/mcp.m111.010694, 1-12, 2012.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	gas chromatography/mass spectrometry ; metabonomic analysis ; mass-spectrometry ; mouse model ; hplc-ms ; liver ; cancer ; progression ; hepatitis ; carcinogenesis
收录类别	SCI
语种	英语
WOS记录号	WOS:000300056200011
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118406]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai, Peoples R China 3.Sixth Peoples Hosp Dalian, Dalian 116031, Peoples R China
推荐引用方式 GB/T 7714	Tan, Yexiong,Yin, Peiyuan,Tang, Liang,et al. Metabolomics Study of Stepwise Hepatocarcinogenesis From the Model Rats to Patients: Potential Biomarkers Effective for Small Hepatocellular Carcinoma Diagnosis[J]. molecular & cellular proteomics,2012,11(2):106941.
APA	Tan, Yexiong.,Yin, Peiyuan.,Tang, Liang.,Xing, Wenbin.,Huang, Qiang.,...&Xu, Guowang.(2012).Metabolomics Study of Stepwise Hepatocarcinogenesis From the Model Rats to Patients: Potential Biomarkers Effective for Small Hepatocellular Carcinoma Diagnosis.molecular & cellular proteomics,11(2),106941.
MLA	Tan, Yexiong,et al."Metabolomics Study of Stepwise Hepatocarcinogenesis From the Model Rats to Patients: Potential Biomarkers Effective for Small Hepatocellular Carcinoma Diagnosis".molecular & cellular proteomics 11.2(2012):106941.