A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine

	A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine
	Long, Zhen 1; Wang, Chaoran 1; Guo, Zhimou 1; Zhang, Xiuli 1; Nordahl, Lilly 1; Zeng, Jing 1,2; Zeng, Jianguo 2; Liang, Xinmiao 1
刊名	analyst
	2012
卷号	137 期号:6 页码:1451-1457
通讯作者	梁鑫淼 ; 张秀莉
产权排序	1,1
英文摘要	a non-aqueous solid phase extraction (spe) method utilizing silica based strong cation exchange (scx) was developed and optimized for the enrichment of alkaloids. in this method, silica based scx spe columns were used for the elimination of non-alkaloid compounds and the preconcentration of alkaloids from the extracts. mass spectrometry was employed to analyze the alkaloid-enriched fraction, and results showed that the spe method developed in this study was effective for the removal of non-alkaloids. then, this pretreatment method was combined with high performance liquid chromatography for the quantification of scopolamine and hyoscyamine from scopolia tangutica maxim. the recoveries of scopolamine and (-)-hyoscyamine were 98.51% and 91.12%, respectively. relative standard deviation values were 1.4% for scopolamine and 1.6% for (-)-hyoscyamine. the linearity was good in the 0.01-0.8 mg ml(-1) range for hyoscyamine and 0.01-0.4 mg ml(-1) range for scopolamine.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	performance liquid-chromatography ; tandem mass-spectrometry ; ion-exchange equilibria ; basic drugs ; pyrrolizidine alkaloids ; mixed-solvents ; hplc ; separation ; eluents ; water
收录类别	SCI
语种	英语
WOS记录号	WOS:000300510900024
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118320]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Liaoning Provin, Peoples R China 2.Hunan Agr Univ, Coll Hort & Gardening, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Long, Zhen,Wang, Chaoran,Guo, Zhimou,et al. A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine[J]. analyst,2012,137(6):1451-1457.
APA	Long, Zhen.,Wang, Chaoran.,Guo, Zhimou.,Zhang, Xiuli.,Nordahl, Lilly.,...&Liang, Xinmiao.(2012).A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine.analyst,137(6),1451-1457.
MLA	Long, Zhen,et al."A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine".analyst 137.6(2012):1451-1457.