Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds

	Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds
	Long, Zhen; Wang, Chaoran; Guo, Zhimou; Zhang, Xiuli; Nordahl, Lilly; Liang, Xinmiao
刊名	journal of chromatography a
	2012-09-21
卷号	1256 页码:67-71
关键词	SCX Basic compounds Symmetrical peaks Load amount Separation efficiency
通讯作者	梁鑫淼 ; 郭志谋
产权排序	1,1
英文摘要	strong cation exchange (scx) mode, an alternative to reversed-phase (rp) mode, was described in this paper to obtain symmetrical peak shape and high sample loading for the separation of basic compounds. the retention mechanisms on scx modified silica sorbents have been demonstrated including electrostatic and hydrophobic interactions. the effects of eluent ionic strength, apparent ph on retention were investigated and the cation-exchange capacity was also characterized. good efficiency (>56,000 plate m(-1)) and excellent peak shape (tf < 1.2) for various basic probes were obtained on the scx column. furthermore, good peak shapes and separation can be maintained under high concentration injections, indicating good potential in preparative use. these results were also compared with those obtained on two conventional rp stationary phases. the overall results demonstrated that scx stationary phases can be used as alternatives to rp stationary phases in the separation of basic compounds, especially in the purification of basic compounds. (c) 2012 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; reversed-phase chromatography ; ion chromatography ; stationary phases ; drugs ; ph ; retention ; system ; enrichment ; alkaloids
收录类别	SCI
语种	英语
WOS记录号	WOS:000308624000009
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/118161]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning Provin, Peoples R China
推荐引用方式 GB/T 7714	Long, Zhen,Wang, Chaoran,Guo, Zhimou,et al. Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds[J]. journal of chromatography a,2012,1256:67-71.
APA	Long, Zhen,Wang, Chaoran,Guo, Zhimou,Zhang, Xiuli,Nordahl, Lilly,&Liang, Xinmiao.(2012).Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds.journal of chromatography a,1256,67-71.
MLA	Long, Zhen,et al."Strong cation exchange column allow for symmetrical peak shape and increased sample loading in the separation of basic compounds".journal of chromatography a 1256(2012):67-71.