The features of an emerging whispovirus isolate from freshwater crayfish

doi:10.1016/j.aqrep.2021.100728

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	The features of an emerging whispovirus isolate from freshwater crayfish
	Ke, Fei 1,2,3; Gui, Lang 4; Li, Tao 1,2,3; Li, Fu-Hua 5; Zhao, Xin 1,2,3; Chen, Zhong-Yuan 1,2,3; Lei, Cun-Ke 1,2,3; Zhang, Qi-Ya 1,2,3,4
刊名	AQUACULTURE REPORTS
	2021-07-01
卷号	20 页码:17
关键词	Nimaviridae Genome architecture Variable region Recombination Freshwater crayfish Cambarus clarkii whispovirus
ISSN号	2352-5134
DOI	10.1016/j.aqrep.2021.100728
通讯作者	Zhang, Qi-Ya(zhangqy@ihb.ac.cn)
英文摘要	A highly lethal freshwater crayfish Cambarus clarkii whispovirus (WSSV-Cc) was isolated from a naturally infected host. A large number of viral particles with typical nimavirus characters were observed in host cells. Genomic architecture and genetic evolution of this extremely virulent pathogen were uncovered. WSSV-Cc has a double-stranded DNA genome containing 287,179 bp, encoding 180 putative ORFs. Congruence analysis was performed between WSSV-Cc and known nimavirus genomes. The genome of WSSV-Cc is smaller than that found in isolates sampled before 2010, but is similar in size to that of recent isolates, which provided new evidence for the shrinkage of nimavirus genome size during evolution. Phylogenetic analysis with the complete genome also showed connections between genome size and evolutionary relationship. Twenty low-similarity genes and three variable regions were identified from the WSSV-Cc genome. In addition, two recombination events were detected in the WSSV-Cc genome, and analysis of the recombinant regions showed a close relationship among the three freshwater crayfish nimaviruses. These findings provide us the morphological, genomic, and genetic characters of an emerging whispovirus, which will broaden our understanding to nimaviruses and be useful for diagnosis of crayfish viral disease.
资助项目	National Key R&D Plan of the Ministry of Science and Technology of China[2018YFD0900302] ; National Key R&D Plan of the Ministry of Science and Technology of China[2020YFD0900303] ; National Natural Science Foundation of China[31772890] ; Strategic Pilot Science and Technology of the Chinese Academy of Sciences Project[XDA24030203] ; Chinese Academy of Sciences[KJZD-SW-L11] ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ04]
WOS研究方向	Fisheries
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000674482200009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/175745]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Qi-Ya
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Coll Modern Agr Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China 4.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Int Res Ctr Marine Biosci,Minist Educ,Minist Sci, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Ke, Fei,Gui, Lang,Li, Tao,et al. The features of an emerging whispovirus isolate from freshwater crayfish[J]. AQUACULTURE REPORTS,2021,20:17.
APA	Ke, Fei.,Gui, Lang.,Li, Tao.,Li, Fu-Hua.,Zhao, Xin.,...&Zhang, Qi-Ya.(2021).The features of an emerging whispovirus isolate from freshwater crayfish.AQUACULTURE REPORTS,20,17.
MLA	Ke, Fei,et al."The features of an emerging whispovirus isolate from freshwater crayfish".AQUACULTURE REPORTS 20(2021):17.