Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation

doi:10.1021/acs.analchem.0c04110

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation
	Wang, Yadi 2,3,4; Dai, Wentao 1,5; Liu, Zhixiao 3; Liu, Jixiang 1; Cheng, Jie 2,3; Li, Yuanyuan 1,5; Li, Xueling 6; Hu, Jun 2,3; Lü, Junhong 2,3
刊名	Analytical Chemistry
	2021
卷号	93 期号:2 页码:671-676
关键词	Stem cells Cell culture Cell proliferation Fourier transform infrared spectroscopy Microoptics Adipogenic differentiations Dynamic heterogeneity FTIR microspectroscopy Human mesenchymal stem cells Infrared micro spectroscopy Mesenchymal stem cell Quantitative evaluation Single cell resolution
ISSN号	0003-2700
DOI	10.1021/acs.analchem.0c04110
文献子类	期刊论文
英文摘要	The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the cell-to-cell similarity distance of the infrared spectral data. Based on this method, we revealed the infrared phenotypes might reflect the dynamic heterogeneity changes in the cell population during the adipogenic differentiation of the human mesenchymal stem cells. These findings provide an alternative label-free optical approach for quantifying the cellular heterogeneity, and the combination with other single-cell analysis tools will be very helpful for understanding the genotype-to-phenotype relationship in cellular populations. © 2020 American Chemical Society.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/33139]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Center for Bioinformation Technology, No.1278 Ke Yuan Road, Pudong New District, Shanghai; 201203, China; 2.Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 239 Zhangheng Road, Pudong New District, Shanghai; 201203, China; 3.Shanghai Institute of Applied Physics, Chinese Academy of Sciences, No. 2019 Jia Luo Road, Jiading District, Shanghai; 201800, China; 4.University of Chinese Academy of Sciences, No.19A Yuquan Road, Shijingshan District, Beijing; 100049, China; 5.Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Huangpu District, Shanghai; 200025, China; 6.Shanghai University of Medicine and Health Sciences, National Engineering Research Center for Nanotechnology, No. 28 Jiangchuan East Road, Minhang District, Shanghai; 201318, China
推荐引用方式 GB/T 7714	Wang, Yadi,Dai, Wentao,Liu, Zhixiao,et al. Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation[J]. Analytical Chemistry,2021,93(2):671-676.
APA	Wang, Yadi.,Dai, Wentao.,Liu, Zhixiao.,Liu, Jixiang.,Cheng, Jie.,...&Lü, Junhong.(2021).Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation.Analytical Chemistry,93(2),671-676.
MLA	Wang, Yadi,et al."Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation".Analytical Chemistry 93.2(2021):671-676.