Programming Switchable Transcription of Topologically Constrained DNA

doi:10.1021/jacs.0c01962

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Programming Switchable Transcription of Topologically Constrained DNA
	Jiao, K ; Au, B ; Guo, LJ ; Zhou, HB ; Wang, F ; Zhang, XL ; Shi, JY ; Li, Q ; Wang, LH ; Li, J
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2020
卷号	142 期号:24 页码:10739-10746
关键词	DELIVERY NANOSTRUCTURES RNA NANOROBOT SHAPE
ISSN号	0002-7863
DOI	10.1021/jacs.0c01962
文献子类	期刊论文
英文摘要	Genomic DNA is compacted via chromatin condensation in mammalian cells, and transcription of such topologically constrained DNA to messenger RNA is under strict spatiotemporal regulation. Nevertheless, control of DNA topology has been poorly explored in in vitro transcription and gene transfection. Here we report the construction of topologically ordered (TO-) prokaryotic genes composed of linear DNA templates appended with a T7 promoter sequence with the use of DNA self-assembly. We find that TO-DNA maintains the transcription activity whereas the activity is critically dependent on the configuration of the T7 promoter in a folded DNA nanostructure. By prescribing the position and the intactness of the T7 promoter, we can dynamically activate or repress transcription in response to specific DNA key strands in a Boolean logic manner. Bioorthogonal switchable transcription is realized with the insertion of multiple genes in a TO-DNA. Further, implementing TO-DNA in living bacteria leads to switchable transcription of fluorescent RNA aptamers for light-up cell imaging. Hence, the design of TO-DNAs provides a means for shape-dependent gene delivery, enriching the toolbox of genetic engineering and synthetic biology.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/33092]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Southern Med Univ, Fengxian Hosp, Guangzhou, Peoples R China 2.East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China 4.Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Zhangjiang Lab,Shanghai Adv Res Inst, Shanghai 201204, Peoples R China 5.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Frontiers Sci Ctr Transformat Mol, Shanghai 200240, Peoples R China 6.Shanghai Jiao Tong Univ, Inst Mol Med, Shanghai Key Lab Nucle Acids Chem & Nanomed, Renji Hosp,Sch Med, Shanghai 200240, Peoples R China 7.Shanghai Jiao Tong Univ, Joint Res Ctr Precis Med, Peoples Hosp 6, South Campus, Shanghai 201499, Peoples R China
推荐引用方式 GB/T 7714	Jiao, K,Au, B,Guo, LJ,et al. Programming Switchable Transcription of Topologically Constrained DNA[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020,142(24):10739-10746.
APA	Jiao, K.,Au, B.,Guo, LJ.,Zhou, HB.,Wang, F.,...&Fan, CH.(2020).Programming Switchable Transcription of Topologically Constrained DNA.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,142(24),10739-10746.
MLA	Jiao, K,et al."Programming Switchable Transcription of Topologically Constrained DNA".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142.24(2020):10739-10746.