Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction

doi:10.1038/s41596-020-0326-4

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction
	Ye, DK ; Li, M ; Zhai, TT ; Song, P ; Song, L ; Wang, H ; Mao, XH ; Wang, F ; Zhang, XL ; Ge, ZL
刊名	NATURE PROTOCOLS
	2020
卷号	15 期号:7 页码:2163-2185
关键词	DNA HYDROGEL BIOSENSING INTERFACE CAPTURE AMPLIFICATION RECOGNITION PLATFORM
ISSN号	1754-2189
DOI	10.1038/s41596-020-0326-4
文献子类	期刊论文
英文摘要	Circulating tumor cells (CTCs) enable noninvasive liquid biopsy and identification of cancer. Various approaches exist for the capture and release of CTCs, including microfluidic methods and those involving magnetic beads or nanostructured solid interfaces. However, the concomitant cell damage and fragmentation that often occur during capture make it difficult to extensively characterize and analyze living CTCs. Here, we describe an aptamer-trigger-clamped hybridization chain reaction (atcHCR) method for the capture of CTCs by porous 3D DNA hydrogels. The 3D environment of the DNA networks minimizes cell damage, and the CTCs can subsequently be released for live-cell analysis. In this protocol, initiator DNAs with aptamer-toehold biblocks specifically bind to the epithelial cell adhesion molecule (EpCAM) on the surface of CTCs, which triggers the atcHCR and the formation of a DNA hydrogel. The DNA hydrogel cloaks the CTCs, facilitating quantification with minimal cell damage. This method can be used to quantitively identify as few as 10 MCF-7 cells in a 2-mu L blood sample. Decloaking of tumor cells via gentle chemical stimulus (ATP) is used to release living tumor cells for subsequent cell culture and live-cell analysis. We also describe how to use the protocol to encapsulate and release cells of cancer cell lines, which can be used in preliminary experiments to model CTCs. The whole protocol takes similar to 2.5 d to complete, including downstream cell culture and analysis.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32848]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med,Dept Lab Med,Shanghai Key Lab Nucle, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil SSRF,CAS Key La, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil SSRF,CAS Key La, Shanghai, Peoples R China 5.Shanghai Jiao Tong Univ, Joint Res Ctr Precis Med, Shanghai, Peoples R China 6.Southern Med Univ, Affiliated Fengxian Hosp, Affiliated Sixth Peoples Hosp, South Campus, Shanghai, Peoples R China 7.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Ye, DK,Li, M,Zhai, TT,et al. Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction[J]. NATURE PROTOCOLS,2020,15(7):2163-2185.
APA	Ye, DK.,Li, M.,Zhai, TT.,Song, P.,Song, L.,...&Zuo, XL.(2020).Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction.NATURE PROTOCOLS,15(7),2163-2185.
MLA	Ye, DK,et al."Encapsulation and release of living tumor cells using hydrogels with the hybridization chain reaction".NATURE PROTOCOLS 15.7(2020):2163-2185.