DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers

doi:10.1021/acs.nanolett.0c01898

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers
	Chen, SX ; Su, J ; Zhao, ZH ; Shao, Y ; Dou, YZ ; Li, FW ; Deng, WP ; Shi, JY ; Li, Q ; Zuo, XL
刊名	NANO LETTERS
	2020
卷号	20 期号:10 页码:7028-7035
ISSN号	1530-6984
DOI	10.1021/acs.nanolett.0c01898
文献子类	期刊论文
英文摘要	Epigenetic alterations hold great promise as biomarkers for early stage cancer diagnosis. Nevertheless, direct identification of rare methylated DNA in the genome remains challenging. Here, we report an ultrasensitive framework nucleic acid-based electrochemical sensor for quantitative and highly selective analysis of DNA methylation. Notably, we can detect 160 fg of methylated DNA in million-fold unmethylated DNA samples using this electrochemical methylation-specific polymerase chain reaction (E-MSP) method. The high sensitivity of E-MSP enables one-step detection of low-abundance methylation at two different genes in patient serum samples. By using a combination test with two methylation alterations, we achieve high accuracy and sensitivity for reliable differentiation of prostate cancer and benign prostate hypertrophy (BPH). This new method sheds new light on translational use in early cancer diagnosis and in monitoring patients' responses to therapeutic agents.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32806]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Shanghai Jiao Tong Univ, Ruijin Hosp North, Sch Med, Dept Urol, Shanghai 201801, Peoples R China 2.Shanghai Jiao Tong Univ, Inst Mol Med, Shanghai Key Lab Nucle Acids Chem & Nanomed, Renji Hosp,Sch Med, Shanghai 200240, Peoples R China 3.Shanghai Jiao Tong Univ, Frontiers Sci Ctr Transformat Mol, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Biomed Engn, Inst Personalized Med, Shanghai 200030, Peoples R China 7.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Chen, SX,Su, J,Zhao, ZH,et al. DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers[J]. NANO LETTERS,2020,20(10):7028-7035.
APA	Chen, SX.,Su, J.,Zhao, ZH.,Shao, Y.,Dou, YZ.,...&Fan, CH.(2020).DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers.NANO LETTERS,20(10),7028-7035.
MLA	Chen, SX,et al."DNA Framework-Supported Electrochemical Analysis of DNA Methylation for Prostate Cancers".NANO LETTERS 20.10(2020):7028-7035.