DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis

doi:10.1021/acs.analchem.9b05616

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis
	Wen, YL ; Li, LY ; Li, J ; Lin, MH ; Liu, G ; Liang, W ; Xu, L ; Li, Y ; Zuo, XL ; Ren, SZ
刊名	ANALYTICAL CHEMISTRY
	2020
卷号	92 期号:6 页码:4498-4503
ISSN号	0003-2700
DOI	10.1021/acs.analchem.9b05616
文献子类	期刊论文
英文摘要	MicroRNAs (miRNAs) have been explored as biomarkers for early diagnosis of diseases like cancers. However, it remains challenging to detect low-level miRNAs in the total RNA from real samples in a facile approach. In this work, we report a two-phase miRNA biosensing strategy based on a modular framework nucleic acid (FNA) platform, which combines the high efficiency of homogeneous reaction and the convenience of heterogeneous biosensing. In the first phase, free DNA probes bind target miRNAs in a homogeneous solution, forming a DNA-RNA complex with high base stacking energy. Then, at the second phase, the universal FNA interface on the electrode selectively mediated the transition of the complex from the solution onto the interface for electrochemical signal generating and transduction. By applying this method, we detected as few as 1 aM of miR-141, a cancer marker miRNA, without the need for nucleic acid amplification. The dynamic range spans 10 orders of magnitude. We demonstrate multiplex miRNA detection and discrimination of highly homologous miRNAs with mismatches as few as a single base. We also show that this system can detect miR-141 in only 50 ng of total RNA samples from real cells, which allows discrimination of prostate cancer cells with normal cells. We envision this platform may satisfy the need for facile and high-throughput screening of early cancer markers.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32805]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Inst Mol Med, Shanghai 200240, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Lab Phys Biol, Shanghai 201800, Peoples R China 5.Shanghai Inst Measurement & Testing Technol, Div Chem & Ionizing Radiat Measurement Technol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wen, YL,Li, LY,Li, J,et al. DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis[J]. ANALYTICAL CHEMISTRY,2020,92(6):4498-4503.
APA	Wen, YL.,Li, LY.,Li, J.,Lin, MH.,Liu, G.,...&Zhu, Y.(2020).DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis.ANALYTICAL CHEMISTRY,92(6),4498-4503.
MLA	Wen, YL,et al."DNA Framework-Mediated Electrochemical Biosensing Platform for Amplification-Free MicroRNA Analysis".ANALYTICAL CHEMISTRY 92.6(2020):4498-4503.