Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry

	Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry
	Zhang, Xihao 1,2; He, Xiwen 1,2; Chen, Langxing 1,2; Zhang, Yukui 1,2,3
刊名	journal of materials chemistry
	2012
卷号	22 期号:32 页码:16520-16526
通讯作者	陈朗星
产权排序	3,4
英文摘要	in this study, a novel approach was developed to synthesize aminophenylboronic acid functionalized magnetic nanoparticles (nps) via cu(i)-catalyzed azide-alkyne cycloaddition (cuaac) 'click' chemistry. firstly, azide-functional fe3o4 nps were obtained by a two-step chemical modification process. then, an alkyne-phenylboronic acid molecule was connected onto the surface of magnetite by the cuaac reaction. the morphology, structure and composition of the synthesized nanocomposites were characterized by transmission electron microscopy (tem), x-ray powder diffraction (xrd), vibrating sample magnetometer (vsm), fourier transform infrared (ftir) spectroscopy and x-ray photoelectron spectrometry (xps). five proteins, including ovalbumin (ob), transferrin (trf), as glycoprotein templates and lysozyme (lyz), bovine serum albumin (bsa), horse heart cytochrome c (cyt c) as nonglycoprotein templates are chosen as target proteins. the as-prepared click-fe3o4@apba nps with a mean diameter of 23.2 nm showed a strong magnetic response to an externally applied magnetic field and exhibited a high adsorption capacity and excellent specificity towards glycoproteins in comparison with nonglycoproteins. the click-fe3o4@apba nps showed the higher adsorption capacity towards glycoproteins than the nonclick-fe3o4@apba nps which were synthesized through a common nucleophilic substitution reaction. the greatly enhanced adsorption capacity towards glycoproteins demonstrated that the 'click' method presented great superiority in ligand immobilization. finally, the click-fe3o4@apba nps could efficiently enrich glycoproteins from real egg white samples as well.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences ; technology
类目[WOS]	chemistry, physical ; materials science, multidisciplinary
研究领域[WOS]	chemistry ; materials science
关键词[WOS]	affinity monolithic column ; iron-oxide nanoparticles ; one-pot synthesis ; selective enrichment ; gold nanoparticles ; protein separation ; mesoporous silica ; facile synthesis ; glycopeptides ; glycosylation
收录类别	SCI
语种	英语
WOS记录号	WOS:000306708700051
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117833]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xihao,He, Xiwen,Chen, Langxing,et al. Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry[J]. journal of materials chemistry,2012,22(32):16520-16526.
APA	Zhang, Xihao,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2012).Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry.journal of materials chemistry,22(32),16520-16526.
MLA	Zhang, Xihao,et al."Boronic acid modified magnetic nanoparticles for enrichment of glycoproteins via azide and alkyne click chemistry".journal of materials chemistry 22.32(2012):16520-16526.