Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS

doi:10.3390/molecules24010034

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 国家天然药物工程技术研究中心 > 天然产物研究

	Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS
	Xue, Ying 1,2,3; Jin, Wei 1; Xu, Xian-Shun 1; Yong, Li 1; Hu, Bin 1; Xiong, Jing 3; Hu, Xue-Mei 3; Qing, Lin-Sen 2,3; Xie, Jing 3
刊名	MOLECULES
	2019
卷号	24 期号:1 页码:34
关键词	Tricholoma matsutake quality evaluation nucleic acid compound UPLC-TOF MS UPLC-QqQ MS
ISSN号	1420-3049
DOI	10.3390/molecules24010034
产权排序	2
文献子类	Article
英文摘要	So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2-deoxyadenosine, 2-deoxycytidine, 2-deoxyguanosine, 2-deoxyuridine, adenosine 5-monophosphate, cytidine 5-monophosphate, guanosine 5-monophosphate, and uridine 5-monophosphate. Then, a UPLC-QqQ/MS method was developed for the subsequent quantitative analysis. After validating the limits of quantification, detection, precision, repeatability, and recovery through a calibration curve, the content of 15 nucleic acid compounds was determined by the proposed UPLC-QqQ/MS method in 80 T. matsutake samples collected from different regions in Sichuan province, Southwest China. After the statistical analysis, we suggest that the total content of nucleic acid compounds in the qualified T. matsutake should be higher than 24.49 mg/100 g. The results indicated that the combined use of UPLC-TOF/MS and UPLC-QqQ/MS is efficient for fast identification and determination of nucleic acid compounds to comprehensively evaluate the quality of T. matsutake.
学科主题	Chemistry
URL标识	查看原文
WOS关键词	IDENTIFICATION ; IONIZATION ; EXTRACTION
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000457150200034
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/31063]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Sichuan Prov Ctr Dis Control & Prevent, Chengdu 610041, Sichuan, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China; 3.Chengdu Med Coll, Sch Pharm, Collaborat Innovat Ctr Sichuan Elderly Care & Hlt, Chengdu 610500, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Xue, Ying,Jin, Wei,Xu, Xian-Shun,et al. Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS[J]. MOLECULES,2019,24(1):34.
APA	Xue, Ying.,Jin, Wei.,Xu, Xian-Shun.,Yong, Li.,Hu, Bin.,...&Xie, Jing.(2019).Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS.MOLECULES,24(1),34.
MLA	Xue, Ying,et al."Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS".MOLECULES 24.1(2019):34.