Ligand fishing of anti-neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B

doi:10.1002/jssc.201801255

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	Ligand fishing of anti-neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B
	Wu, Gui-Fang 1,2; Jiang, Xiao-Lan 1,2; Gong, Yu-Zhou 1,2; Hu, Ya-Dong 2; Bai, Xiao-Lin 2; Liao, Xun 2
刊名	JOURNAL OF SEPARATION SCIENCE
	2019
卷号	42 期号:6 页码:1289-1298
关键词	enzyme inhibitors Lonicera japonica magnetic nanoparticles monoamine oxidase B neurodegenerative diseases
ISSN号	1615-9306
DOI	10.1002/jssc.201801255
产权排序	1
文献子类	Article
英文摘要	In this work, monoamine oxidase B was immobilised onto magnetic nanoparticles to prepare a new type of affinity solid-phase extraction adsorbent, which was used to extract the possible anti-neurodegenerative components from the Lonicera japonica flower extracts. Coupled with high-performance liquid chromatography with mass spectrometry, two monoamine oxidase B ligands were fished-out and identified as isochlorogenic acid A and isochlorogenic acid C, which were found to be inhibitors of the enzyme for the first time, with similar half maximal inhibitory concentration values of 29.05 +/- 0.49 and 29.77 +/- 1.03 mu M, respectively. Furthermore, equilibrium-dialysis dissociation assay of enzyme-inhibitor complex showed that both compounds have reversible binding patterns to monoamine oxidase B, and kinetic analysis demonstrated that they were mixed-type inhibitors for monoamine oxidase B, with K-i and K-is values of 9.55 and 37.24 mu M for isochlorogenic acid A, 9.53 and 35.50 mu M for isochlorogenic acid C, respectively. The results indicated that isochlorogenic acid A and isochlorogenic acid C were the major active components responsible for the anti-degenerative activity of the flowers of L. japonica, while magnetic nanoparticles immobilised monoamine oxidase B could serve as an efficient solid-phase extraction adsorbent to specifically extract monoamine oxidase B inhibitors from complex herbal extracts.
学科主题	Chemistry & Analysis
URL标识	查看原文
WOS关键词	FLOWER BUDS ; MAO-A ; ACID ; INHIBITORS ; CHROMATOGRAPHY ; PHARMACOLOGY ; EXTRACTION ; SAMPLES ; UPDATE ; ENZYME
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000462257700018
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30531]
专题	国家天然药物工程技术研究中心_天然产物研究
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Chengdu Inst Biol, 9,Sect 4,South Renmin Rd, Chengdu, Sichuan, Peoples R China;
推荐引用方式 GB/T 7714	Wu, Gui-Fang,Jiang, Xiao-Lan,Gong, Yu-Zhou,et al. Ligand fishing of anti-neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B[J]. JOURNAL OF SEPARATION SCIENCE,2019,42(6):1289-1298.
APA	Wu, Gui-Fang,Jiang, Xiao-Lan,Gong, Yu-Zhou,Hu, Ya-Dong,Bai, Xiao-Lin,&Liao, Xun.(2019).Ligand fishing of anti-neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B.JOURNAL OF SEPARATION SCIENCE,42(6),1289-1298.
MLA	Wu, Gui-Fang,et al."Ligand fishing of anti-neurodegenerative components from Lonicera japonica using magnetic nanoparticles immobilised with monoamine oxidase B".JOURNAL OF SEPARATION SCIENCE 42.6(2019):1289-1298.