Transcription of mcrA Gene Decreases Upon Prolonged Non-flooding Period in a Methanogenic Archaeal Community of a Paddy-Upland Rotational Field Soil

doi:10.1007/s00248-017-1063-2

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 生物多样性与生态系统服务领域 > 中国科学院山地生态恢复与生物资源利用重点实验室

	Transcription of mcrA Gene Decreases Upon Prolonged Non-flooding Period in a Methanogenic Archaeal Community of a Paddy-Upland Rotational Field Soil
	Liu, Dongyan 2,3; Nishida, Mizuhiko 1; Takahashi, Tomoki 1; Asakawa, Susumu 3
刊名	MICROBIAL ECOLOGY
	2018-04-01
卷号	75 期号:3 页码:751-760
关键词	Methanogenic archaea mcrA transcripts CH4 Paddy-upland rotation
ISSN号	0095-3628
DOI	10.1007/s00248-017-1063-2
产权排序	2
文献子类	Article
英文摘要	Methanogenic archaea survive under aerated soil conditions in paddy fields, and their community is stable under these conditions. Changes in the abundance and composition of an active community of methanogenic archaea were assessed by analyzing mcrA gene (encoding alpha subunit of methyl-coenzyme M reductase) and transcripts during a prolonged drained period in a paddy-upland rotational field. Paddy rice (Oryza sativa L.) was planted in the flooded field and rotated with soybean (Glycine max [L.] Merr.) under upland soil conditions. Soil samples were collected from the rotational plot in the first year, with paddy rice, and in the two successive years, with soybean, at six time points, before seeding, during cultivation, and after harvest as well as from a consecutive paddy (control) plot. By the time that soybean was grown in the second year, the methanogenic archaeal community in the rotational plot maintained high mcrA transcript levels, comparable with those of the control plot community, but the levels drastically decreased by over three orders of magnitude after 2 years of upland conversion. The composition of active methanogenic archaeal communities that survived upland conversion in the rotational plot was similar to that of the active community in the control plot. These results revealed that mcrA gene transcription of methanogenic archaeal community in the rotational field was affected by a prolonged non-flooding period, longer than 1 year, indicating that unknown mechanisms maintain the stability of methanogenic archaeal community in paddy fields last up to 1 year after the onset of drainage.
学科主题	Environment ; Ecology
URL标识	查看原文
WOS关键词	RIBOSOMAL-RNA ; METHANE EMISSIONS ; FOREST SOILS ; RICE-WHEAT ; BACTERIA ; DESICCATION ; POPULATIONS ; SENSITIVITY ; DYNAMICS ; CATALASE
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000427696000018
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30292]
专题	生物多样性与生态系统服务领域_中国科学院山地生态恢复与生物资源利用重点实验室
作者单位	1.NARO Tohoku Agricultural Research Center,Daisen, Akita 014-0120, Japan ; 2.CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province,Chengdu Institute of Biology, Chinese Academy of Sciences,Chengdu 610041, China; 3.Nagoya Univ, Grad Sch Bioagr Sci, Soil Biol & Chem, Chikusa Ku, Nagoya, Aichi 4648601, Japan; 4.NARO Tohoku Agr Res Ctr, Daisen, Akita 0140120, Japan
推荐引用方式 GB/T 7714	Liu, Dongyan,Nishida, Mizuhiko,Takahashi, Tomoki,et al. Transcription of mcrA Gene Decreases Upon Prolonged Non-flooding Period in a Methanogenic Archaeal Community of a Paddy-Upland Rotational Field Soil[J]. MICROBIAL ECOLOGY,2018,75(3):751-760.
APA	Liu, Dongyan,Nishida, Mizuhiko,Takahashi, Tomoki,&Asakawa, Susumu.(2018).Transcription of mcrA Gene Decreases Upon Prolonged Non-flooding Period in a Methanogenic Archaeal Community of a Paddy-Upland Rotational Field Soil.MICROBIAL ECOLOGY,75(3),751-760.
MLA	Liu, Dongyan,et al."Transcription of mcrA Gene Decreases Upon Prolonged Non-flooding Period in a Methanogenic Archaeal Community of a Paddy-Upland Rotational Field Soil".MICROBIAL ECOLOGY 75.3(2018):751-760.