The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes

doi:10.1186/s12864-019-5939-z

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes
	Zhou, Min(1m,2,3); Zheng, Shigang 1; Liu, Rong 1,2; Lu, Lu 1; Zhang, Chihong 1; Zhang, Lei 1; Yant, Levi 3; Wu, Yu 1
刊名	BMC GENOMICS
	2019
卷号	20 页码:615
关键词	Wheat RNA sequencing microRNA HMA
ISSN号	1471-2164
DOI	10.1186/s12864-019-5939-z
产权排序	1
文献子类	Article
英文摘要	BackgroundHeavy metal ATPases (HMAs) are responsible for Cd translocation and play a primary role in Cd detoxification in various plant species. However, the characteristics of HMAs and the regulatory mechanisms between HMAs and microRNAs in wheat (Triticum aestivum L) remain unknown.ResultsBy comparative microRNA and transcriptome analysis, a total three known and 19 novel differentially expressed microRNAs (DEMs) and 1561 differentially expressed genes (DEGs) were found in L17 after Cd treatment. In H17, by contrast, 12 known and 57 novel DEMs, and only 297 Cd-induced DEGs were found. Functional enrichments of DEMs and DEGs indicate how genotype-specific biological processes responded to Cd stress. Processes found to be involved in microRNAs-associated Cd response include: ubiquitin mediated proteolysis, tyrosine metabolism, and carbon fixation pathways and thiamine metabolism. For the mRNA response, categories including terpenoid backbone biosynthesis and phenylalanine metabolism, and photosynthesis - antenna proteins and ABC transporters were enriched. Moreover, we identified 32 TaHMA genes in wheat. Phylogenetic trees, chromosomal locations, conserved motifs and expression levels in different tissues and roots under Cd stress are presented. Finally, we infer a microRNA-TaHMAs expression network, indicating that miRNAs can regulate TaHMAs.ConclusionOur findings suggest that microRNAs play important role in wheat under Cd stress through regulation of targets such as TaHMA2;1. Identification of these targets will be useful for screening and breeding low-Cd accumulation wheat lines.
学科主题	Biology
URL标识	查看原文
WOS关键词	P-TYPE ATPASE ; TO-SHOOT TRANSLOCATION ; DOWN-REGULATION ; HEAVY-METALS ; HEALTH-RISK ; ARABIDOPSIS ; TRANSPORT ; ZINC ; ACCUMULATION ; TOLERANCE
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BMC
WOS记录号	WOS:000477825600005
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30939]
专题	环境治理与食品安全领域_农业生物技术研究
通讯作者	Wu, Yu
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Univ Nottingham, Sch Life Sci, Nottingham NG7 2RD, England
推荐引用方式 GB/T 7714	Zhou, Min,Zheng, Shigang,Liu, Rong,et al. The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes[J]. BMC GENOMICS,2019,20:615.
APA	Zhou, Min.,Zheng, Shigang.,Liu, Rong.,Lu, Lu.,Zhang, Chihong.,...&Wu, Yu.(2019).The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes.BMC GENOMICS,20,615.
MLA	Zhou, Min,et al."The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes".BMC GENOMICS 20(2019):615.