Tomato SIAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins

doi:10.1038/s41438-018-0032-3

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 农业生物技术研究

	Tomato SIAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins
	Gao, Yongfeng 3; Liu, Jikai 3; Chen, Yongfu 3; Tang, Hai 3; Wang, Yang 3; He, Yongmei 4; Ou, Yongbin 3; Sun, Xiaochun 1; Wang, Songhu 2; Yao, Yinan 3
刊名	HORTICULTURE RESEARCH
	2018
卷号	5 页码:27
ISSN号	2052-7276
DOI	10.1038/s41438-018-0032-3
产权排序	4
文献子类	Article
英文摘要	The flavonoid compounds are important secondary metabolites with versatile human nutritive benefits and fulfill a multitude of functions during plant growth and development. The abundance of different flavonoid compounds are finely tuned with species-specific pattern by a ternary MBW complex, which consists of a MYB, a bHLH, and a WD40 protein, but the essential role of SIAN11, which is a WD40 protein, is not fully understood in tomato until now. In this study, a tomato WD40 protein named as SIAN11 was characterized as an effective transcription regulator to promote plant anthocyanin and seed proanthocyanidin (PA) contents, with late flavonoid biosynthetic genes activated in 35S::SIAN77 transgenic lines, while the dihydroflavonol flow to the accumulation of flavonols or their glycosylated derivatives was reduced by repressing the expression of SIFLS in this SIAN/1-overexpressed lines. The above changes were reversed in 35S::SIAN77-RNAi transgenic lines except remained levels of flavonol compounds and SIFLS expression. Interestingly, our data revealed that SIAN11 gene could affect seed dormancy by regulating the expressions of abscisic acid (ABA) signaling-related genes SIABI3 and SIABI5, and the sensitivity to ABA treatment in seed germination is conversely changed by SIAN/ 1-overexpressed or -downregulated lines. Yeast two-hybrid assays demonstrated that SIAN11 interacted with bHLH but not with MYB proteins in the ternary MBW complex, whereas bHLH interacted with MYB in tomato. Our results indicated that low level of anthocyanins in tomato fruits, with low expression of bHLH (SITT8) and MYB (SIANT1 and SIAN2) genes, remain unchanged upon modification of SIAN11 gene alone in the transgenic lines. These results suggest that the tomato WD40 protein SIAN11, coordinating with bHLH and MYB proteins, plays a crucial role in the fine adjustment of the flavonoid biosynthesis and seed dormancy in tomato.
学科主题	Plant Sciences
URL标识	查看原文
WOS关键词	WD40 REPEAT PROTEIN ; ARABIDOPSIS-THALIANA ; TRANSCRIPTION FACTOR ; ABSCISIC-ACID ; PROANTHOCYANIDIN BIOSYNTHESIS ; TRICHOME DIFFERENTIATION ; ANTHOCYANIN BIOSYNTHESIS ; DOMAIN PROTEIN ; GENE ENCODES ; CELL FATE
WOS研究方向	Plant Sciences ; Agriculture
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000433920100001
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30704]
专题	环境治理与食品安全领域_农业生物技术研究
作者单位	1.Shaanxi Univ Chinese Med, Shaanxi Collaborat Innovat Ctr Chinese Med Resour, Xianyang 712046, Peoples R China; 2.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China 3.Southwest Univ Sci & Technol, Sch Life Sci & Engn, Mianyang 621010, Peoples R China; 4.Innovat Team Farmland Nonpollut Prod Yunnan Prov, Yunnan Engn Lab Agroenvironm Pollut Control & Eco, Kunming 650201, Yunnan, Peoples R China;
推荐引用方式 GB/T 7714	Gao, Yongfeng,Liu, Jikai,Chen, Yongfu,et al. Tomato SIAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins[J]. HORTICULTURE RESEARCH,2018,5:27.
APA	Gao, Yongfeng.,Liu, Jikai.,Chen, Yongfu.,Tang, Hai.,Wang, Yang.,...&Yao, Yinan.(2018).Tomato SIAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins.HORTICULTURE RESEARCH,5,27.
MLA	Gao, Yongfeng,et al."Tomato SIAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins".HORTICULTURE RESEARCH 5(2018):27.