Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix

	Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix
	Zhao, Yanyan 1,2; Li, Xiuling 1; Yan, Jingyu 1; Guo, Zhimou 1; Liang, Xinmiao 1
刊名	analytical methods
	2012-05-01
卷号	4 期号:5 页码:1244-1251
ISSN号	1759-9660
通讯作者	李秀玲 ; 梁鑫淼
产权排序	1,1
英文摘要	reversible protein phosphorylation regulates many significant cellular processes. identification of phosphorylation sites is vital to elucidate their biofunctions. to aid in phosphoproteome characterization, several selective enrichment methods have been developed, including immobilized metal-ion affinity chromatography (imac) and titanium dioxide (tio2). however, the high ph elution step applied in these two methods, which tends to degrade phosphopeptides. in order to improve phosphopeptide enrichment efficiency, a hydrophilic interaction liquid chromatography (hilic) based material, cyclodextrin (cd) bonded silica (click oeg-cd), was synthesized in our group and applied to phosphopeptide enrichment and fractionation. taking tryptic digest of standard protein a-casein as model sample, the performance of click oeg-cd in phosphopeptide isolation was investigated. it was found that both the acetonitrile and salt concentrations in mobile phase influence the phosphopeptide enrichment selectivity of the matrix. under optimized enrichment condition, click oeg-cd has a similar phosphopeptide enrichment selectivity as commercial tio2. meanwhile, phosphopeptides with same charges could be fractionated according to hydrophilicity difference on click oeg-cd under acetonitrile gradient. different selectivity is proved with click oeg-cd from conventional sax. we demonstrate that click oeg-cd is an efficient matrix in phosphopeptide enrichment and fractionation.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences ; life sciences & biomedicine ; technology
类目[WOS]	chemistry, analytical ; food science & technology ; spectroscopy
研究领域[WOS]	chemistry ; food science & technology ; spectroscopy
关键词[WOS]	interaction liquid-chromatography ; metal affinity-chromatography ; highly selective enrichment ; phosphoproteome analysis ; phosphorylated peptides ; mass-spectrometry ; titanium-dioxide ; exchange ; anion ; identification
收录类别	SCI
语种	英语
WOS记录号	WOS:000303603000009
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117720]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Med Univ, Coll Pharm, Dalian 116044, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yanyan,Li, Xiuling,Yan, Jingyu,et al. Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix[J]. analytical methods,2012,4(5):1244-1251.
APA	Zhao, Yanyan,Li, Xiuling,Yan, Jingyu,Guo, Zhimou,&Liang, Xinmiao.(2012).Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix.analytical methods,4(5),1244-1251.
MLA	Zhao, Yanyan,et al."Phosphopeptide enrichment and fractionation by using Click OEG-CD matrix".analytical methods 4.5(2012):1244-1251.