Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase

doi:10.1002/cbic.201800236

CORC > 成都生物研究所 > 中国科学院成都生物研究所 > 食品安全与环境治理领域 > 应用与环境微生物研究

	Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase
	Xie, Tian 1,2; Liu, Zhongchuan 1,2; Wang, Ganggang 1,2
刊名	CHEMBIOCHEM
	2018
卷号	19 期号:14 页码:1502-1506
关键词	allosterism copper enzymes hydrogen bonds structure elucidation
ISSN号	1439-4227
DOI	10.1002/cbic.201800236
产权排序	1
文献子类	Article
英文摘要	In laccase, type1 copper (Cu1) was connected to the trinuclear copper center (TNC) by the conserved Cys-His bridge. An allosteric coupling between the two redox sites has been reported; however, the molecular mechanism underlining the allosteric coupling is unknown. In this study, ligands of the two type3 copper sites, including His491 and His493, in CotA were mutated to Cys or Ala. The crystal structures revealed that mutations at His491 and His493 caused rearrangement of the hydrogen-bond network and geometric distortion of the TNC, which severely impaired the activities of mutants H493A, H493C, and H491C. In addition, the change in TNC affected hydrogen bonds around Cys492 in the mutants and led to Cu1 being partially reduced. These results not only decipher the mechanism of allosteric coupling between Cu1 and TNC in laccase, but also pave the way for laccase protein engineering.
学科主题	Chemistry ; Analysis
URL标识	查看原文
WOS关键词	MULTICOPPER OXIDASE FET3P ; CRYSTAL-STRUCTURE ; SUBSTRATE ; OXIDATION ; MUTANTS ; O-2
WOS研究方向	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000439494900008
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/30678]
专题	环境治理与食品安全领域_应用与环境微生物研究
作者单位	1.Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Sichuan, Peoples R China; 2.Chinese Acad Sci, Key Lab Environm Microbiol Sichuan Prov, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China
推荐引用方式 GB/T 7714	Xie, Tian,Liu, Zhongchuan,Wang, Ganggang. Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase[J]. CHEMBIOCHEM,2018,19(14):1502-1506.
APA	Xie, Tian,Liu, Zhongchuan,&Wang, Ganggang.(2018).Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase.CHEMBIOCHEM,19(14),1502-1506.
MLA	Xie, Tian,et al."Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase".CHEMBIOCHEM 19.14(2018):1502-1506.