Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells

	Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells
	Liu, Yang 1; Liu, Chang 1,2; Nan, Feng 3; Xiao, Jing 4; Lian, Jian-chun 5; He, Xin 5; Guo, Xin 1; Sun, Guang-wei 1; Ma, Xiao-jun 1
刊名	journal of hard tissue biology
	2013-04-01
卷号	22 期号:2 页码:241-247
关键词	Cell co-culture Mesenchymal stem cell Microcapsule Neural differentiation
通讯作者	孙广炜 ; 马小军
产权排序	1,1
英文摘要	cell co-culture plays a key role in inducing the committed differentiation of stem cells in vitro, which mimics the specific tissue microenvironment in vivo by means of co-cultured inducing cells. however, the present cell co-culture systems limit the application of differentiated cells due to the safety and the scale of these systems. this study established a novel microcapsule co-culture system and investigated the probable effect of microencapsulated co-cultured neural cells on neural differentiation of mesenchymal stem cells (mscs) ex vivo. mouse bone marrow-derived mscs were co-cultured with shsy5y cells microencapsulated in alginate-polylysine-alginate (apa) microcapsules. the quantitative and qualitative analysis showed that the expression of nestin (nes) was significantly increased at both gene and protein levels in the microcapsule co-culture system compared with other systems without cell co-culture, which indicated that more neural progenitors were obtained from this novel microcapsule co-culture system. according to our results and the unique characteristics of microcapsules, it is possible that large-scale cell co-culture system based on the combination of microcapsule and bioreactor technologies will be developed to generate numerous mscs-derived neural progenitors for clinical applications in the future.
学科主题	物理化学
WOS标题词	science & technology ; technology
类目[WOS]	engineering, biomedical
研究领域[WOS]	engineering
关键词[WOS]	transplantation ; expression ; therapy ; culture ; stroke ; growth ; injury ; rat
收录类别	SCI
语种	英语
WOS记录号	WOS:000318190200013
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117649]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Dalian Med Univ, Hosp 2, Dept Orthoped, Dalian, Peoples R China 4.Dalian Med Univ, Collage Stomatol, Dept Oral Basic Sci, Dalian, Peoples R China 5.Dalian Med Univ, Lab Med Coll, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yang,Liu, Chang,Nan, Feng,et al. Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells[J]. journal of hard tissue biology,2013,22(2):241-247.
APA	Liu, Yang.,Liu, Chang.,Nan, Feng.,Xiao, Jing.,Lian, Jian-chun.,...&Ma, Xiao-jun.(2013).Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells.journal of hard tissue biology,22(2),241-247.
MLA	Liu, Yang,et al."Microcapsule Co-Culture System Enhances Neural Differentiation of Mesenchymal Stem Cells".journal of hard tissue biology 22.2(2013):241-247.