A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum

	A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum
	Zhu, Jun 1; Wang, Fangjun 1; Cheng, Kai 1; Dong, Jing 1; Sun, Deguang 2; Chen, Rui 1; Wang, Liming 2; Ye, Mingliang 1; Zou, Hanfa 1
刊名	proteomics
	2013-04-01
卷号	13 期号:8 页码:1306-1313
关键词	ACN-improved digestion Filter-aided sample preparation Glycoproteomics Human serum Sialic acid-containing N-glycopeptide Ti(IV)-IMAC
ISSN号	1615-9853
通讯作者	邹汉法
产权排序	1,1
英文摘要	terminal sialylation is very important in cancer biology and has been extensively investigated for the discovery of potential clinical biomarkers of cancers. in this study, we presented a novel approach, by using of ti(iv)-imac, to enrich sialic-acid-containing n-glycopeptides for the analysis of terminal sialylation. compared with conventional method using tio2, this approach obtained 2.5 times more glycopeptides and glycosylation sites. then, a simple integrated system combining filter-aided sample preparation, acn-improved digestion, and ti(iv)-imac enrichment was established for efficient analysis. in this system, protein digestion, glycopeptide enrichment, and deglycosylation were integrated and were performed sequentially in a single filter unit without any need for desalting, lyophilization, or sample transfer procedures. as a result, the number of identifications was improved by 1.5-fold and the total processing time was drastically reduced to only 78 h. by using this system, fast and efficient analysis of human serum sialylated n-glycoproteome was achieved. from only 1 l of human serum, 217 unique glycopeptides and 194 glycosylation sites were successfully identified.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	mass-spectrometry ; proteome analysis ; aberrant glycosylation ; enrichment ; chromatography ; identification ; solubilization ; proteins ; plasma
收录类别	SCI
语种	英语
WOS记录号	WOS:000317684800008
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117616]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Jun,Wang, Fangjun,Cheng, Kai,et al. A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum[J]. proteomics,2013,13(8):1306-1313.
APA	Zhu, Jun.,Wang, Fangjun.,Cheng, Kai.,Dong, Jing.,Sun, Deguang.,...&Zou, Hanfa.(2013).A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum.proteomics,13(8),1306-1313.
MLA	Zhu, Jun,et al."A simple integrated system for rapid analysis of sialic-acid-containing N-glycopeptides from human serum".proteomics 13.8(2013):1306-1313.