Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model

	Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model
	Xu, Xiao-xi 1,2; Liu, Chang 1,2; Liu, Yang 1; Li, Nan 1,2; Guo, Xin 1; Wang, Shu-jun 1,3; Sun, Guang-wei 1; Wang, Wei 1; Ma, Xiao-jun 1
刊名	experimental cell research
	2013-08-15
卷号	319 期号:14 页码:2135-2144
关键词	Alginate bead Metastasis model Tumor microenvironment Matrix metalloproteinase Invasion
通讯作者	孙广炜 ; 马小军
产权排序	1,1
英文摘要	hepatocellular carcinoma (hcc) is the most common primary liver cancer and often forms metastases, which are the most important prognostic factors. for further elucidation of the mechanism underlying the progression and metastasis of hcc, a culture system mimicking the in vivo tumor microenvironment is needed. in this study, we investigated the metastatic ability of hcc cells cultured within alginate gel (alg) beads. in the culture system, hcc cells formed spheroids by proliferation and maintained in nuclear abnormalities. the gene and protein expression of metastasis-related molecules was increased in alg beads, compared with the traditional adhesion culture. furthermore, several gene expression levels in alg bead culture system were even closer to liver cancer tissues. more importantly, in vitro invasion assay showed that the invasion cells derived from alg beads was 7.8-fold higher than adhesion cells. our results indicated that the in vitro three-dimensional (3d) model based on alg beads increased metastatic ability compared with adhesion culture, even partly mimicked the in vivo tumor tissues. moreover, due to the controllable preparation conditions, steady characteristics and production at large-scale, the 3d alg bead model would become an important tool used in the high-throughput screening of anti-metastasis drugs and the metastatic mechanism research. (c) 2013 elsevier inc. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	oncology ; cell biology
研究领域[WOS]	oncology ; cell biology
关键词[WOS]	matrix metalloproteinases ; mesenchymal transition ; bioartificial pancreas ; tumor spheroids ; culture-system ; hepg2 cells ; cancer ; invasion ; tissue ; 3d
收录类别	SCI
语种	英语
WOS记录号	WOS:000322804900002
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117606]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiao-xi,Liu, Chang,Liu, Yang,et al. Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model[J]. experimental cell research,2013,319(14):2135-2144.
APA	Xu, Xiao-xi.,Liu, Chang.,Liu, Yang.,Li, Nan.,Guo, Xin.,...&Ma, Xiao-jun.(2013).Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model.experimental cell research,319(14),2135-2144.
MLA	Xu, Xiao-xi,et al."Encapsulated human hepatocellular carcinoma cells by alginate gel beads as an in vitro metastasis model".experimental cell research 319.14(2013):2135-2144.