In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer

	In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer
	Li, Kai 1,2; Sun, Zhen 3; Zheng, Jianyong 1,2; Lu, Yuanyuan 1,2; Bian, Yangyang 3; Ye, Mingliang 3; Wang, Xin 1,2; Nie, Yongzhan 1,2; Zou, Hanfa 3; Fan, Daiming 1,2
刊名	journal of proteomics
	2013-04-26
卷号	82 页码:130-140
关键词	Biomarker Cell surface capturing Gastric cancer Glycoproteomics Multidrug resistance
ISSN号	1874-3919
通讯作者	邹汉法 ; daimingfan
产权排序	2,2
英文摘要	human gastric cancer is a big public health problem. multidrug resistance is a main obstacle to successful chemotherapeutic treatment in gastric cancers and the underlying mechanism is not clear. glycosylation, one of the most important post translational modifications of proteins, plays a vital role in diverse aspects of tumor progression. in the present study, we applied two multidrug resistance cell lines and their parental drug sensitive gastric cancer cell line to a modified cell surface capturing strategy with triplex labeling to characterize mdr related cell surface glycoproteome. finally, 56 cell membrane glycoproteins were successfully identified via combination of identification by glycopeptides and quantitation by non-glycopeptides, and 11 of them were found to be differentially expressed with the same trend in both drug resistant cell lines compared with that in sensitive cell line. the further analysis by western blot and in vitro drug sensitivity assay demonstrated that our approach is reliable and accurate and suggested that these glycoproteins may represent as biomarkers for multidrug resistance in gastric cancer.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	n-linked glycosylation ; quantitative proteomics ; ovarian-carcinoma ; drug-resistance ; p-glycoprotein ; expression ; identification ; serum ; lines ; quantification
收录类别	SCI
语种	英语
WOS记录号	WOS:000319643300010
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117604]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Fourth Mil Med Univ, State Key Lab Canc Biol, Xian 710032, Shaanxi Provinc, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Shaanxi Provinc, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Li, Kai,Sun, Zhen,Zheng, Jianyong,et al. In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer[J]. journal of proteomics,2013,82:130-140.
APA	Li, Kai.,Sun, Zhen.,Zheng, Jianyong.,Lu, Yuanyuan.,Bian, Yangyang.,...&Fan, Daiming.(2013).In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer.journal of proteomics,82,130-140.
MLA	Li, Kai,et al."In-depth research of multidrug resistance related cell surface glycoproteome in gastric cancer".journal of proteomics 82(2013):130-140.