A six-plex proteome quantification strategy reveals the dynamics of protein turnover

	A six-plex proteome quantification strategy reveals the dynamics of protein turnover
	Wang, Fangjun; Cheng, Kai; Wei, Xiaoluan; Qin, Hongqiang; Chen, Rui; Liu, Jing; Zou, Hanfa
刊名	scientific reports
	2013-05-10
卷号	3 页码:18271
ISSN号	2045-2322
通讯作者	王方军 ; 邹汉法
产权排序	1,1
英文摘要	ms1 full scan based quantification is one of the most popular approaches for large-scale proteome quantification. typically only three different samples can be differentially labeled and quantified in a single experiment. here we present a two stages stable isotope labeling strategy which allows six different protein samples (six-plex) to be reliably labeled and simultaneously quantified at ms1 level. briefly in the first stage, isotope lysine-d0 (k0) and lysine-d4 (k4) are in vivo incorporated into different protein samples during cell culture. then in the second stage, three of k0 and k4 labeled protein samples are digested by lysine c and in vitro labeled with light (2ch(3)), medium (2cd(2)h), and heavy (2(13)cd(3)) dimethyl groups, respectively. we demonstrated that this six-plex isotope labeling strategy could successfully investigate the dynamics of protein turnover in a high throughput manner.
学科主题	物理化学
WOS标题词	science & technology
类目[WOS]	multidisciplinary sciences
研究领域[WOS]	science & technology - other topics
关键词[WOS]	multiplexed quantitative proteomics ; mass-spectrometry ; peptide identification ; accurate ; cells ; expression
收录类别	SCI
语种	英语
WOS记录号	WOS:000318652900004
公开日期	2013-10-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/117587]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fangjun,Cheng, Kai,Wei, Xiaoluan,et al. A six-plex proteome quantification strategy reveals the dynamics of protein turnover[J]. scientific reports,2013,3:18271.
APA	Wang, Fangjun.,Cheng, Kai.,Wei, Xiaoluan.,Qin, Hongqiang.,Chen, Rui.,...&Zou, Hanfa.(2013).A six-plex proteome quantification strategy reveals the dynamics of protein turnover.scientific reports,3,18271.
MLA	Wang, Fangjun,et al."A six-plex proteome quantification strategy reveals the dynamics of protein turnover".scientific reports 3(2013):18271.