14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)

doi:10.1016/j.dci.2021.104176

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	14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)
	Xue, Rui 1,2,3; Yang, Dinglong 1,2,3; Han, Yijing 1,4; Deng, Qinyou 5; Wang, Xin 1,4; Liu, Xiangquan 5; Zhao, Jianmin 1,2,3
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2021-11-01
卷号	124 页码:12
关键词	14-3-3 zeta 14-3-3 epsilon Immune responses Pacific abalone
ISSN号	0145-305X
DOI	10.1016/j.dci.2021.104176
通讯作者	Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
英文摘要	The 14-3-3 proteins play important roles in various cellular processes by binding to different ligands, but little is known about these proteins in mollusks. In this study, two 14-3-3 cDNAs were identified from the Pacific abalone Haliotis discus hannai (designated 14-3-3 zeta and 14-3-3 epsilon), possessing 59.40% identity with each other. Both genes were predominantly expressed in the gills of unchallenged abalones, and their mRNA signals could also be detected in several other tissues, including the mantle, hepatopancreas and ovary. However, after Vibrio harveyi challenge, hemocytes were induced significantly (p < 0.01). Meanwhile, phagocytosis was inhibited, but apoptosis, reactive oxygen species formation, and caspase 3 expression were significantly induced (p < 0.01), and they were all suppressed with 14-3-3 zeta knockdown (p < 0.01). The differences were that silencing 14-3-3 epsilon reverted the decline in the phagocytic rate derived from bacterial infection, while ROS formation was not influenced significantly. In addition, the expression levels of several antimicrobial peptide and proinflammatory cytokine genes were also decreased with the silencing of 14-3-3 genes. However, with the knockdown of 14-3-3 zeta, the expression of 14-3-3 epsilon was further significantly increased (p < 0.01), and vice versa. Overall, our results suggested that 14-3-3 zeta and 14-3-3 epsilon should play important roles in innate immunity against V. harveyi infection.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050303] ; National Natural Science Foundation of China[41806196] ; Natural Science Foundation of Shandong Province[ZR2019BD022] ; Yantai Science and Technology Development Project[2020MSGY066] ; Youth Innovation Promotion Association of CAS[2019216]
WOS关键词	CELL-CYCLE CONTROL ; EXPRESSION ANALYSIS ; VIBRIO-HARVEYI ; ALPHA-SYNUCLEIN ; PROTEINS ; 14-3-3-PROTEINS ; IDENTIFICATION ; APOPTOSIS ; FAMILY ; TUBERCULATA
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种	英语
WOS记录号	WOS:000692706800002
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Association of CAS
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29734]
专题	中国科学院牟平海岸带环境综合试验站烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室海岸带生物资源高效利用研究与发展中心
通讯作者	Yang, Dinglong; Zhao, Jianmin
作者单位	1.Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264117, Shandong, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Xue, Rui,Yang, Dinglong,Han, Yijing,et al. 14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,124:12.
APA	Xue, Rui.,Yang, Dinglong.,Han, Yijing.,Deng, Qinyou.,Wang, Xin.,...&Zhao, Jianmin.(2021).14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai).DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,124,12.
MLA	Xue, Rui,et al."14-3-3 zeta and 14-3-3 epsilon are involved in innate immune responses in Pacific abalone (Haliotis discus hannai)".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 124(2021):12.