Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene

doi:10.1016/j.marpolbul.2021.112552

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene
	Yuan, Xiutang 1,2; Zhao, Huan 3; Wang, Yinan 2,3; Wang, Lili 2; Li, Dongmei 2; Zhang, Anguo 2; Yang, Xiaolong 2; Ma, Xindong 2; Yang, Dazuo 3; Zhou, Yibing 3
刊名	MARINE POLLUTION BULLETIN
	2021-08-01
卷号	169 页码:6
关键词	Polychaete PAH biotransformation GST Liaohe Estuary
ISSN号	0025-326X
DOI	10.1016/j.marpolbul.2021.112552
通讯作者	Yuan, Xiutang(xtyuan@yic.ac.cn)
英文摘要	Polychaete worms can eliminate polycyclic aromatic hydrocarbons (PAHs) in environments through a mechanism that increases their water solubility. This detoxification starts with cytochrome P450 enzymes (CYPs) and then with glutathione S-transferases (GSTs). Here, a novel GST gene was identified and characterized from the widespread polychaete Perinereis aibuhitensis. The full-length cDNA of GST is 1544 bp and encodes 256 amino acids, belonging to the omega class. Gene expression patterns in P. aibuhitensis showed that its transcriptional level was positively correlated with the concentration of benzo[alpha]pyrene (0.5, 2, 4, and 8 mu g/L) exposure but was negatively correlated with a PAH benzene ring after it was exposed to the same mass concentrations of fluoranthene (3.2 mu g/L), phenanthrene (2.9 mu g/L), and benzo[alpha]pyrene (4.0 mu g/L) during the 14-day experimentation. These findings indicate that omega GST may play an important role in the phase II detoxification of PAHs in polychaete worms, and the persistence and bioavailability of PAHs may depend on benzene rings.
资助项目	National Natural Science Foundation of China[41106115] ; National Natural Science Foundation of China[41306138] ; National Marine Public Welfare Research Project[201305043]
WOS关键词	POLYCYCLIC AROMATIC-HYDROCARBONS ; MOLECULAR-CLONING ; PAHS ; METABOLITES ; SEDIMENT ; ESTUARY ; NUNTIA
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000679313100012
资助机构	National Natural Science Foundation of China ; National Marine Public Welfare Research Project
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29545]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_近岸生态与环境实验室
通讯作者	Yuan, Xiutang
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Minist Ecol & Environm, Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Xiutang,Zhao, Huan,Wang, Yinan,et al. Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene[J]. MARINE POLLUTION BULLETIN,2021,169:6.
APA	Yuan, Xiutang.,Zhao, Huan.,Wang, Yinan.,Wang, Lili.,Li, Dongmei.,...&Zhou, Yibing.(2021).Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene.MARINE POLLUTION BULLETIN,169,6.
MLA	Yuan, Xiutang,et al."Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene".MARINE POLLUTION BULLETIN 169(2021):6.