Staining of subfossil chironomid head capsules: a method for improving the extraction process from lake sediments and peat

doi:10.1007/s10750-020-04469-7

	Staining of subfossil chironomid head capsules: a method for improving the extraction process from lake sediments and peat
	Cao, Yanmin 1; Zheng, Zijie 1,4; Luo, Xuan 1,2; Langdon, Peter G.3; Dai, Zhe 1; Zhou, Anqi 1
刊名	HYDROBIOLOGIA
	2021-01-02
页码	10
关键词	Subfossil chironomids Staining Aniline blue Cotton blue Method improvement
ISSN号	0018-8158
DOI	10.1007/s10750-020-04469-7
通讯作者	Cao, Yanmin(caoyanmin0720@126.com)
英文摘要	Subfossil chironomids are regarded as a useful biological proxy for palaeoenvironmental research, but picking chironomid head capsules from aquatic sediments is extremely time-consuming. This is often the case for finding and isolating small head capsules from the deflocculated sediment. In this study, two stains (aniline blue and cotton blue) were used to dye chironomid head capsules in lake and peat sediments to improve the traditional pretreatment method and boost the picking efficiency. The results suggested that: (1) there were sharp contrasts between chironomids and residues after staining, which could shorten picking time for both lake and peat samples; (2) different parts of head capsules showed distinctive colour in stained samples and thus facilitated identification and taxonomy of subfossil chironomids; (3) the dyeing effects of two stains were comparable and no significant effect of staining on chironomid composition has been observed compared with unstained samples. This study demonstrates that aniline blue and cotton blue stain can promote picking efficiency of subfossil chironomids, and can be widely applied to pretreatment of sedimentary chironomids in palaeoenvironmental studies on lakes and peatlands.
资助项目	National Natural Science Foundation of China[41877428] ; National Natural Science Foundation of China[41402307] ; Fundamental Research Funds for National University, South-Central University for Nationalities[CZZ18007]
WOS关键词	HYPOLIMNETIC OXYGEN ; INFERENCE MODEL ; AIR-TEMPERATURE ; ANILINE BLUE ; DIPTERA ; LARVAE ; RECONSTRUCTION ; COMMUNITIES ; INDICATORS ; EFFICIENCY
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000604160900004
资助机构	National Natural Science Foundation of China ; Fundamental Research Funds for National University, South-Central University for Nationalities
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/32486]
专题	中国科学院广州能源研究所
通讯作者	Cao, Yanmin
作者单位	1.South Cent Univ Nationalities, Coll Resources & Environm Sci, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 3.Univ Southampton, Sch Geog & Environm Sci, Southampton SO17 1BJ, Hants, England 4.Beijing Normal Univ, Sch Environm, State Key Lab Environm Simulat, Beijing 100875, Peoples R China
推荐引用方式 GB/T 7714	Cao, Yanmin,Zheng, Zijie,Luo, Xuan,et al. Staining of subfossil chironomid head capsules: a method for improving the extraction process from lake sediments and peat[J]. HYDROBIOLOGIA,2021:10.
APA	Cao, Yanmin,Zheng, Zijie,Luo, Xuan,Langdon, Peter G.,Dai, Zhe,&Zhou, Anqi.(2021).Staining of subfossil chironomid head capsules: a method for improving the extraction process from lake sediments and peat.HYDROBIOLOGIA,10.
MLA	Cao, Yanmin,et al."Staining of subfossil chironomid head capsules: a method for improving the extraction process from lake sediments and peat".HYDROBIOLOGIA (2021):10.