E2F1/2/4 mRNA is associated with immune infiltration and are potential biomarkers for the prognosis of human gastric carcinoma

doi:10.21037/tcr-21-45

	E2F1/2/4 mRNA is associated with immune infiltration and are potential biomarkers for the prognosis of human gastric carcinoma
	Chen, Yongyi 1,2; Gong, Wangang 2,3; Dai, Wumin 2,3; Jiang, Huifen 1,2; Xu, Xiaohong 1,2
刊名	TRANSLATIONAL CANCER RESEARCH
	2021-06-01
卷号	10
关键词	E2F1/2/4 gastric cancer (GC) immune infiltration bioinformatics analysis
ISSN号	2218-676X
DOI	10.21037/tcr-21-45
通讯作者	Xu, Xiaohong(zjhzxxh@163.com)
英文摘要	Background: E2Fs are genes that regulate DNA synthesis and the cell cycle by encoding a family of transcription factors. Increasing experimental evidence has revealed that E2Fs play key roles in tumor progression in various types of cancer. Methods: We investigated the survival, expression and transcriptional data of E2F1/2/4 in gastric cancer (GC) patients using the immunohistochemistry assay, Kaplan-Meier Plotter, cBioPortal, String, and GEPIA databases. The plasma of GC patients was analyzed using the real-time reverse transcription polymerase chain reaction (RT- PCR) assay. The correlation between E2F1/2/4 expression and clinical features was analyzed using the quartile method. As well, the correlation between E2F1/2/4 and GC immune infiltration was also investigated using the TIMER database. Database of Immune Cell Expression (DICE) was also used to analyze correlations between SOX4 and immune responses. Results: RT- PCR and tissue immunohistochemistry confirmed that E2F1/2/4 was highly expressed in serum and GC tissue samples of GC patients, the expression of which was not affected by patient age and gender. Also, the survival analysis revealed that low levels of E2F1/ 2/4 expression were significantly associated with a longer overall survival (OS) in GC patients. E2F1/2/4 was correlated with patient prognosis and immune cell infiltration, including B cells, CD8(+) T cells, CD4(+) T cells, macrophages, neutrophils, and DCs in GC. Our findings indicated that E2F1/2/4 could be used as a prognostic biomarker and indicator of immune infiltration in GC. Conclusions: This study revealed that E2F1/2/4 could be a promising indicator for tumor-associated immune infiltration and prognosis in GC patients.
资助项目	Zhejiang Province Public Welfare Technology Application Research Project[2021KY082]
WOS关键词	SQUAMOUS-CELL CARCINOMA ; TRANSCRIPTION FACTORS ; CLINICAL-RELEVANCE ; E2F FAMILY ; CANCER ; EXPRESSION ; GENE ; RISK
WOS研究方向	Oncology
语种	英语
出版者	AME PUBL CO
WOS记录号	WOS:000694025800015
资助机构	Zhejiang Province Public Welfare Technology Application Research Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/125305]
专题	中国科学院合肥物质科学研究院
通讯作者	Xu, Xiaohong
作者单位	1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Clin Lab, Hangzhou, Peoples R China 2.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Hangzhou, Peoples R China 3.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Zhejiang Canc Res Inst, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yongyi,Gong, Wangang,Dai, Wumin,et al. E2F1/2/4 mRNA is associated with immune infiltration and are potential biomarkers for the prognosis of human gastric carcinoma[J]. TRANSLATIONAL CANCER RESEARCH,2021,10.
APA	Chen, Yongyi,Gong, Wangang,Dai, Wumin,Jiang, Huifen,&Xu, Xiaohong.(2021).E2F1/2/4 mRNA is associated with immune infiltration and are potential biomarkers for the prognosis of human gastric carcinoma.TRANSLATIONAL CANCER RESEARCH,10.
MLA	Chen, Yongyi,et al."E2F1/2/4 mRNA is associated with immune infiltration and are potential biomarkers for the prognosis of human gastric carcinoma".TRANSLATIONAL CANCER RESEARCH 10(2021).