Influence of the PM2.5 Water-Soluble Compound on the Biophysical Properties of A549 Cells

doi:10.1021/acs.langmuir.1c00522

	Influence of the PM2.5 Water-Soluble Compound on the Biophysical Properties of A549 Cells
	Tang, Mingjie 1,2; Wang, Yan 1,2; Tang, Dongyun 1,2; Xiu, Peng 3; Yang, Zhongbo 1,2; Chen, Yang 2; Wang, Huabin 1,2
刊名	LANGMUIR
	2021-04-06
卷号	37 期号:13 页码:4042-4048
ISSN号	0743-7463
DOI	10.1021/acs.langmuir.1c00522
通讯作者	Xiu, Peng(xiupeng2011@zju.edu.cn) ; Wang, Huabin(wanghuabin@cigit.ac.cn)
英文摘要	Understanding the influence of fine atmospheric particles (PM2.5) on cellular biophysical properties is an integral part for comprehending the mechanisms underlying PM2.5-induced diseases because they are closely related to the behaviors and functions of cells. However, hitherto little work has been done in this area. In the present work, we aimed to interrogate the influence of the PM2.5 water-soluble compound (PM2.5-WSC) on the biophysical performance of a human lung carcinoma epithelial cell line (A549) by exploring the cellular morphological and mechanical changes using atomic force microscopy (AFM)-based imaging and nanomechanics. AFM imaging showed that PM2.5-WSC treated cells exhibited evidently reduced lamellipodia and an increased height when compared to the control group. AFM nanomechanical measurements indicated that the treated cells had higher elastic energy and lower adhesion work than the control group. Our western blot assay and transmission electron microscopy (TEM) results revealed that after PM2.5-WSC treatment, the contents of cytoskeletal components (beta-actin and beta-tubulin) increased, but the abundance of cell surface microvilli decreased. The biophysical changes of PM2.5-WSC-treated cells measured by AFM can be well correlated to the alterations of the cytoskeleton and surface microvilli identified by the western blot assay and TEM imaging. The above findings confirm that the adverse risks of PM2.5 on cells can be reliably assessed biophysically by characterizing the cellular morphology and nanomechanics. The demonstrated technique can be used to diminish the gap of our understanding between PM2.5 and its harmful effects on cellular functions.
资助项目	National Natural Science Foundation of China[U1932132] ; National Natural Science Foundation of China[11932017] ; National Natural Science Foundation of China[11574268] ; National Natural Science Foundation of China[61775213] ; National Natural Science Foundation of China[61875196] ; National Natural Science Foundation of China[12074208] ; University of Chinese Academy of Sciences Supported Program for Tackling Key Problems in Science and Technology[E029610601] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0405] ; Natural Science Foundation of Chongqing[cstc2019jcyj-msxmX0654]
WOS研究方向	Chemistry ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000639003400025
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/13326]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Xiu, Peng; Wang, Huabin
作者单位	1.Chongqing Engn Res Ctr High Resolut & Three Dimen, Chongqing 400714, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Zhejiang Univ, Dept Engn Mech, Hangzhou 310027, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Tang, Mingjie,Wang, Yan,Tang, Dongyun,et al. Influence of the PM2.5 Water-Soluble Compound on the Biophysical Properties of A549 Cells[J]. LANGMUIR,2021,37(13):4042-4048.
APA	Tang, Mingjie.,Wang, Yan.,Tang, Dongyun.,Xiu, Peng.,Yang, Zhongbo.,...&Wang, Huabin.(2021).Influence of the PM2.5 Water-Soluble Compound on the Biophysical Properties of A549 Cells.LANGMUIR,37(13),4042-4048.
MLA	Tang, Mingjie,et al."Influence of the PM2.5 Water-Soluble Compound on the Biophysical Properties of A549 Cells".LANGMUIR 37.13(2021):4042-4048.