Analysis with biological nanopore: On-pore, off-pore strategies and application in biological fluids

doi:10.1016/j.talanta.2020.121684

	Analysis with biological nanopore: On-pore, off-pore strategies and application in biological fluids
	Wang, Yunjiao 2,3; Zhang, Youwen 1; Chen, Xiaohan 1; Guan, Xiyun 1; Wang, Liang 2,3
刊名	TALANTA
	2021-02-01
卷号	223 页码:12
关键词	Nanopore On-pore strategies Off-pore strategies Molecular interactions Single-molecule analysis Biomarkers
ISSN号	0039-9140
DOI	10.1016/j.talanta.2020.121684
通讯作者	Guan, Xiyun(xguan5@iit.edu) ; Wang, Liang(wangliang83@cigit.edu.cn)
英文摘要	Inspired from ion channels in biology, nanopores have been developed as promising analytical tools. In principle, nanopores provide crucial information from the observation and analysis of ionic current modulations caused by the interaction between target analytes and fluidic pores. In this respect, the biological, chemical and physical parameters of the nanopore regime need to be well-understood and regulated for intermolecular interaction. Because of well-defined molecular structures, biological nanopores consequently are of a focal point, allowing precise interaction analysis at single-molecule level. In this overview, two analytical strategies are summarized and discussed accordingly, upon the challenges arising in case-dependent analysis using biological nanopores. One kind of strategies relies on modification, functionalization and engineering on nanopore confined interface to improve molecular recognition sites (on-pore strategies); The other kind of highlighted strategies concerns to measurement of various chemistry/biochemistry based interactions triggered by employed molecular agents or probes (off-pore strategies). In particularly, a few recent paradigms using these strategies for practical application of accurate analysis of biomarkers in biological fluids are illustrated. To end, the challenging and future outlook of using analytical tools by means of biological nanopores are depicted.
资助项目	National Natural Science Foundation of China[31800711] ; Pioneer Hundred Talents Program of Chinese Academy of Sciences ; Chongqing Talents[CQYC201905007] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0304] ; Entrepreneurship and Innovation Support Program for Returned Oversea Students of Chongqing, China[cx2018156] ; National Science Foundation[1708596] ; National Institutes of Health[2R15GM110632-02] ; University of Chinese Academy of Sciences
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000599674700001
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/12652]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Guan, Xiyun; Wang, Liang
作者单位	1.IIT, Dept Chem, Chicago, IL 60616 USA 2.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yunjiao,Zhang, Youwen,Chen, Xiaohan,et al. Analysis with biological nanopore: On-pore, off-pore strategies and application in biological fluids[J]. TALANTA,2021,223:12.
APA	Wang, Yunjiao,Zhang, Youwen,Chen, Xiaohan,Guan, Xiyun,&Wang, Liang.(2021).Analysis with biological nanopore: On-pore, off-pore strategies and application in biological fluids.TALANTA,223,12.
MLA	Wang, Yunjiao,et al."Analysis with biological nanopore: On-pore, off-pore strategies and application in biological fluids".TALANTA 223(2021):12.