Carbon Nanomaze for Biomolecular Detection with Zeptomolar Sensitivity

doi:10.1002/adfm.202006521

	Carbon Nanomaze for Biomolecular Detection with Zeptomolar Sensitivity
	Bao, Jing 1,4; Qiu, Xiaopei 4; Wang, Deqiang 5; Yang, Huisi 1; Zhao, Jiaying 1; Qi, Yanli 1; Zhang, Liangliang 1,2; Chen, Xiaohui 1,2; Yang, Mei 1; Gu, Wei 1,2
刊名	ADVANCED FUNCTIONAL MATERIALS
	2020-12-02
页码	13
关键词	carbon nanomaze DNA tetrahedron electrochemical sensing trace biomolecules
ISSN号	1616-301X
DOI	10.1002/adfm.202006521
通讯作者	Huo, Danqun(huodq@cqu.edu.cn) ; Luo, Yang(luoy@cqu.edu.cn) ; Hou, Changjun(houcj@cqu.edu.cn)
英文摘要	Despite numerous efforts, the accurate determination of trace biomolecules with zeptomolar sensitivity remains elusive. Here, a 3D carbon nanomaze (CAM) electrode for the ultrasensitive detection of trace biomolecules such as nucleic acids, proteins, and extracellular vesicles is reported. The CAM electrode consists of an interlaced carbon fiber array on which intercrossed graphene sheets are vertically tethered in situ, permitting local confinement of trace molecules to increase molecular hybridization efficiency. Furthermore, a self-assembled DNA tetrahedron array adopts a rigid spatial conformation to guarantee the controllable arrangement of immobilized biological probes, facilitating analytical sensitivity and reproducibility. In a proof-of-concept experiment on detecting microRNA-155, a linearity of 0.1 aM to 100 nM and a sensitivity of 0.023 aM (23 zM) are achieved. With the optimal parameters, the proposed nanoelectrode demonstrates encouraging consistency with quantitative real-time polymerase chain reaction during clinical sample detection. Through simple functionalization by appending various biomolecular probes of interest, the developed CAM platform with ultrahigh sensitivity can be exploited as a versatile tool in environmental, chemistry, biology, and healthcare fields.
资助项目	National Natural Science Foundation of China[81772290] ; National Natural Science Foundation of China[81601854] ; National Natural Science Foundation of China[82002251] ; National Natural Science Foundation of China[81871733] ; Graduate Research and Innovation Foundation of Chongqing, China[CYB20070] ; Fundamental Research Funds for the Central Universities[2019CDYGZD007] ; Fundamental Research Funds for the Central Universities[2019CDYGZD005] ; Higher Education Teaching Reform Research Project of Chongqing[203249] ; Chongqing Graduate Tutor Team Construction Project ; Analytical and Testing Center of Chongqing University ; sharing fund of Chongqing University's large equipment
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000594820000001
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/12609]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Huo, Danqun; Luo, Yang; Hou, Changjun
作者单位	1.Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Bioengn Coll,Minist Educ, Chongqing 400044, Peoples R China 2.Chongqing Univ, Ctr Smart Lab & Mol Med, Med Sch, Chongqing 400044, Peoples R China 3.Chongqing Univ, Sch Microelect & Commun Engn, Chongqing Key Lab Biopercept & Intelligent Inform, Chongqing 400044, Peoples R China 4.Third Mil Med Univ, Southwest Hosp, Dept Clin Lab Med, Army Med Univ, Chongqing 400038, Peoples R China 5.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Beijing 400714, Peoples R China
推荐引用方式 GB/T 7714	Bao, Jing,Qiu, Xiaopei,Wang, Deqiang,et al. Carbon Nanomaze for Biomolecular Detection with Zeptomolar Sensitivity[J]. ADVANCED FUNCTIONAL MATERIALS,2020:13.
APA	Bao, Jing.,Qiu, Xiaopei.,Wang, Deqiang.,Yang, Huisi.,Zhao, Jiaying.,...&Hou, Changjun.(2020).Carbon Nanomaze for Biomolecular Detection with Zeptomolar Sensitivity.ADVANCED FUNCTIONAL MATERIALS,13.
MLA	Bao, Jing,et al."Carbon Nanomaze for Biomolecular Detection with Zeptomolar Sensitivity".ADVANCED FUNCTIONAL MATERIALS (2020):13.