Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell

doi:10.1364/BOE.400487

	Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell
	Tang, Mingjie 3,4; Zhang, Mingkun 4; Xia, Liangping 1,4; Yang, Zhongbo 4; Yan, Shihan 4; Wang, Huabin 4; Wei, Dongshan 2,4; Du, Chunlei 4; Cui, Hong-Liang 4
刊名	BIOMEDICAL OPTICS EXPRESS
	2020-09-01
卷号	11 期号:9 页码:5362-5372
ISSN号	2156-7085
DOI	10.1364/BOE.400487
通讯作者	Wei, Dongshan(dswei@dgut.edu.cn)
英文摘要	Many human genetic diseases are caused by single-base mutation in the gene sequence. Since DNA molecules with single-base mutation are extremely difficult to differentiate, existing detection methods are invariably complex and time-consuming. We propose a new label-free and fast terahertz (THz) spectroscopic technique based on a home-made terahertz attenuated total reflection (ATR) microfluidic cell and a terahertz time-domain spectroscopy (THz-TDS) system to detect single-base-mutated DNA molecules. The detected object DNA molecules are normal hemoglobin gene, sickle cell anemia gene (15 nt), JAK2 gene wild type and JAK2 V617F gene mutation (39 nt) from sickle cell anemia and thrombocytopenia, respectively. Results show that the oligonucleotide fragments with single-base mutation can be identified by THz spectroscopy combined with the ATR microfluidic cell, and the recognition effect of short oligonucleotide fragments with single-base mutation is better than that of long oligonucleotide fragments. The terahertz biosensor is shown to have high sensitivity and can be used to detect DNA molecules directly in the solution environment. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
资助项目	National Natural Science Foundation of China[61771138] ; National Natural Science Foundation of China[61775213] ; National Natural Science Foundation of China[61875196]
WOS研究方向	Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	OPTICAL SOC AMER
WOS记录号	WOS:000577455500036
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/11907]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Wei, Dongshan
作者单位	1.Yangtze Normal Univ, Key Lab Micro Nano Optoelect Devices & Intelligen, Chongqing 408100, Peoples R China 2.Dongguan Univ Technol, Sch Elect Engn, Dongguan 523808, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
推荐引用方式 GB/T 7714	Tang, Mingjie,Zhang, Mingkun,Xia, Liangping,et al. Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell[J]. BIOMEDICAL OPTICS EXPRESS,2020,11(9):5362-5372.
APA	Tang, Mingjie.,Zhang, Mingkun.,Xia, Liangping.,Yang, Zhongbo.,Yan, Shihan.,...&Cui, Hong-Liang.(2020).Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell.BIOMEDICAL OPTICS EXPRESS,11(9),5362-5372.
MLA	Tang, Mingjie,et al."Detection of single-base mutation of DNA oligonucleotides with different lengths by terahertz attenuated total reflection microfluidic cell".BIOMEDICAL OPTICS EXPRESS 11.9(2020):5362-5372.