Disposable 3D GNAs/AuNPs DNA-Circuit Strip for miRNAs Dynamic Quantification

doi:10.1002/smll.202001416

	Disposable 3D GNAs/AuNPs DNA-Circuit Strip for miRNAs Dynamic Quantification
	Bao, Jing 2; Qiu, Xiaopei 3; Yang, Huisi 2; Lu, Wenqiang 4; Yang, Mei 2; Gu, Wei 2,5; Wu, Lixiang 6,7,8; Huo, Danqun 2; Luo, Yang 1,5; Hou, Changjun 2,9
刊名	SMALL
	2020-08-31
页码	9
关键词	DNA-circuit strips duplex-specific nuclease enzyme dynamic quantification graphene nanoarrays microRNAs
ISSN号	1613-6810
DOI	10.1002/smll.202001416
通讯作者	Huo, Danqun(huodq@cqu.edu.cn) ; Luo, Yang(luoy@cqu.edu.cn) ; Hou, Changjun(houcj@cqu.edu.cn)
英文摘要	Real-time quantitative monitoring of miRNAs plays an essential role in diagnosis and therapeutics. Herein, a DSN-coupled graphene nanoarray/gold nanoparticles (GNAs/AuNPs) carbon paper (CP) electrode for the dynamic, sensitive, and real-time analysis of miRNAs is reported. GNAs are vertically grown on the conductive CP by radio frequency plasma enhanced chemical vapor deposition, and AuNPs are electrodeposited on CP/GNAs to build a 3D ultrasensitive sensing interface with large specific surface area, good conductivity and biocompatibility. The dynamic quantitative monitoring of microRNA-21 (miR-21) is realized by cyclic voltammetry with a series of different concentrations within 16 min, and this 3D GNAs/AuNPs DNA-circuit strip shows good performance for the simultaneous detection of miR-21 and miR-155, and the detection limits are as low as 21.4 and 30.3 am, respectively. Moreover, comparable detection results are achieved for clinical samples between the proposed sensor and qRT-PCR, suggesting the reliability of the constructed sensor. This ultrasensitive sensing and disposable DNA-circuit strip with 3D structure can efficiently shorten the diffusion distance between reactive biomolecules and the sensing interface, enhance the hybridization of probes and improve the sensitivity of the biosensor, holding great promise for the rapid, quantitative and dynamic monitoring of multiple low concentrations of biomolecules in point-of-care clinical analysis.
资助项目	National Natural Science Foundation of China[81772290] ; National Natural Science Foundation of China[81601854] ; National Natural Science Foundation of China[81871733] ; Graduate Research and Innovation Foundation of Chongqing, China[CYB17037] ; Chongqing science and technology commission[CSTC2018jcyjAX0062] ; Fundamental Research Funds for the Central Universities[2019CDYGZD007] ; Fundamental Research Funds for the Central Universities[2019CDYGZD005] ; Chongqing Graduate Tutor Team Construction Project and Analytical and Testing Center of Chongqing University ; Chongqing University
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000564019000001
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/11650]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Huo, Danqun; Luo, Yang; Hou, Changjun
作者单位	1.Southwest Med Univ, Affiliated Hosp, Dept Nucl Med, Nucl Med & Mol Imaging Key Lab Sichuan Prov, Luzhou 646000, Sichuan, Peoples R China 2.Chongqing Univ, Key Lab Biorheol Sci & Technol, State & Local Joint Engn Lab Vasc Implants, Minist Educ,Bioengn Coll, Chongqing 400044, Peoples R China 3.Third Mil Med Univ, Army Med Univ, Southwest Hosp, Dept Clin Lab, Chongqing 400038, Peoples R China 4.Chinese Acad Sci, Chongqing Key Lab Multiscale Mfg Technol, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 5.Chongqing Univ, Med Sch, Ctr Smart Lab & Mol Med, Chongqing 400044, Peoples R China 6.Chongqing Univ, Dept Clin Lab, Canc Hosp, 181 Han Yu Rd, Chongqing 400030, Peoples R China 7.Chongqing Canc Inst, 181 Han Yu Rd, Chongqing 400030, Peoples R China 8.Chongqing Canc Hosp, 181 Han Yu Rd, Chongqing 400030, Peoples R China 9.Chongqing Univ, Chongqing Key Lab Biopercept & Intelligent Inform, Sch Microelect & Commun Engn, Chongqing 400044, Peoples R China
推荐引用方式 GB/T 7714	Bao, Jing,Qiu, Xiaopei,Yang, Huisi,et al. Disposable 3D GNAs/AuNPs DNA-Circuit Strip for miRNAs Dynamic Quantification[J]. SMALL,2020:9.
APA	Bao, Jing.,Qiu, Xiaopei.,Yang, Huisi.,Lu, Wenqiang.,Yang, Mei.,...&Hou, Changjun.(2020).Disposable 3D GNAs/AuNPs DNA-Circuit Strip for miRNAs Dynamic Quantification.SMALL,9.
MLA	Bao, Jing,et al."Disposable 3D GNAs/AuNPs DNA-Circuit Strip for miRNAs Dynamic Quantification".SMALL (2020):9.