Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina

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	Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina
	Shen, Xin 1,2; Tian, Mei 1; Meng, Xueping 1; Liu, Huilian 3; Cheng, Hanliang 1; Zhu, Changbao 1; Zhao, Fangqing 2
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2012-09-01
卷号	7 期号:3 页码:248-253
关键词	Bryozoa Cheilostomatida Next-generation sequencing Molecular phylogeny Mitochondrial genome
ISSN号	1744-117X
通讯作者	Shen, X (reprint author), Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China.
英文摘要	Next-generation sequencing (NGS) has proven a valuable platform for fast and easy obtaining of large numbers of sequences at relatively low cost. In this study we use shot-gun sequencing method on Illumina HiSeq 2000, to obtain enough sequences for the assembly of the bryozoan Membranipora grandicella (Bryozoa: Cheilostomatida) mitochondrial genome, which is the first representative of the suborder Malacostegina. The complete mitochondrial genome is 15,861 bp in length, which is relatively larger than other studied bryozoans. The mitochondrial genome contains 13 protein-coding genes, 2 ribosomal RNAs and 20 transfer RNAs. To investigate the phylogenetic position and the inner relationships of the phylum Biyozoa, phylogenetic trees were constructed with amino acid sequences of 11 PCGs from 30 metazoans. Two superclades of protostomes, namely Lophotrochozoa and Ecdysozoa, are recovered as monophyletic with strong support in both ML and Bayesian analyses. Somewhat to surprise, Bryozoa appears as the sister group of Chaetognatha with moderate or high support. The relationship among five bryozoans is Tubulipora flabellaris + (M. grandicella + (Flustrellidra hispida + (Bugula neritina + Watersipora subtorquata))), which supports for the view that Cheilostomatida is not a natural, monophyletic clade. NGS proved to be a quick and easy method for sequencing a complete mitochondrial genome. (C) 2012 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Genetics & Heredity
收录类别	SCI
原文出处	10.1016/j.cbd.2012.03.003
语种	英语
WOS记录号	WOS:000308115900003
公开日期	2013-09-25
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12532]
专题	海洋研究所_海洋生物分类与系统演化实验室
作者单位	1.Huaihai Inst Technol, Coll Marine Sci, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Shen, Xin,Tian, Mei,Meng, Xueping,et al. Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2012,7(3):248-253.
APA	Shen, Xin.,Tian, Mei.,Meng, Xueping.,Liu, Huilian.,Cheng, Hanliang.,...&Zhao, Fangqing.(2012).Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,7(3),248-253.
MLA	Shen, Xin,et al."Complete mitochondrial genome of Membranipora grandicella (Bryozoa: Cheilostomatida) determined with next-generation sequencing: The first representative of the suborder Malacostegina".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 7.3(2012):248-253.