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题名	微藻细胞培养与采收及其耦合研究
作者	曾文炉
学位类别	博士
答辩日期	2002
授予单位	中国科学院过程工程研究所
授予地点	中国科学院过程工程研究所
导师	欧阳藩
关键词	微藻 螺旋藻 聚球藻 光生物反应器 细胞培养 气浮分离 泡载分离 耦合
其他题名	Coupling Studies on Microalgae Culturing and Harvesting
学位专业	生物化工
中文摘要	该文运用"化学反应-分离"耦合原理,就微藻工业化生产过程中的细胞培养、生物量采收及其耦合等三方面进行了初步的研究,以期为提高生产效率、降低成本提供新的途径和思路.(1)微藻细胞的培养.(2)微藻细胞生物量采收.(3)微藻细胞培养与采收过程的耦合.
英文摘要	By applying the coupling theory of "Chemical Reaction and in situ Product Removal", this article emphasizes mainly on three aspects of large-scale culture of microalgae, i.e. cell culture, biomass harvest, and coupling both of them, in order to provide new ways for increasing the productivity and lowing the cost. (1) SVIicroalgai ceil culturing Under conditions of batch culture, bubbling CO2 gas at an appropriate flux was beneficial to increase the final cell concentration and to stabilize the pH of culture system. The concentration of total carbon decreased exponentially with the increase of cell concentration. The light attenuation was related to the cell concentration and optical length, and Lambert-Beer law could well depict their relationships. The relationship between cell density and culture time was in accordance with Logistic model, t=lO l.36h was the turning point of growth curve at which the cell growth rate reached its maximum value (0.387 g dry weight/d/L), and t=69.78h and t=l33.06h were turning points among lag growth, exponential growth and stationary phase of the cell growth. Under the steady state of continuous culture, both the total carbon concentration and average light intensity increased with the increase of dilution rate, but the cell concentration decreased with dilution rate increase. The curves of cell productivity and overall cell growth yield against carbon and light energy verse the dilution rate exhibited peak values. The maximum cell growth rate was 0.362 g dry weight/L/d, the optimum dilution rate and cell concentration were 0.465 l/d and 0.769 g dry weight/L respectively. The cell growth yield against carbon and light energy were 1.206 g/g and 6.944 x 100 g/kJ respectively, and the carbon demand for the cell growth was 0.286 g/L/d. The relationships among specific growth rate (1/h ), cell concentration (OD value), total carbon (mg/L) and average light intensity (lx) under batch culture were: The relationships among cell concentration (OD value), cell productivity (g dry weight/L/d) and dilution rate (1/d) under continuous culture were: 2.028 x 10~7( D - 0.078) (D - 0.007)(0.593 -219.252 V (0.080 - D) (D- 6.005") -115.998 D) (2) Microalcjal ceil harvesting Flocculation behavior could be improved by adjusting the pH of the algal suspensio to 11.0-12.5. The flotation tower used with a higher aspect ratio was more beneficial to the performance of dissolved air flotation ( DAF ). The cell recovery in batch DAF increased with increasing air-solids ratio, saturation pressure and saturation time. The cell recovery in continuous DAF increased with increasing the feed ratio of saturation water to cell suspension, saturation pressure and residence time, but it decreased with the increase of feed concentration. The addition of low concentration of surfactant Tween 20 could lower both the surface tension and the diameter of bubbles, which made it possible to harvest microalgal cells under low saturation pressure. The overall mathematical model for continuous DAF was: factor and enrichment factor of batch foam separation verse flow rate of carrying gas and ethanol concentration, the curves of cell recovery verse pH of cell suspension, and the curves of separation factor and enrichment factor verse Tween 20 concentration exhibited a peak value respectively. But the curves of separation factor and enrichment factor verse pH, and the curves of cell recovery verse Tween 20 concentration showed a positive correlativity. The curves of cell recovery and enrichment factor of continuous foam separation verse flow rate of carrying gas, ethanol concentration, pH, feed rate and ionic strength of cell suspension exhibited peak values as well, but they correlated reversely with feed concentration. (3) Coupling ceil cuituring with its harvesting By theoretical analysis of coupling system, the integrating kinetic models of cell concentration, cell productivity, cell recovery and enrichment factor were constructed. The effects of operation parameters such as dilution rate, enrichment factor and recycle ratio of culture medium on carbon concentration, average light intensity, cell concentration and cell productivity were evaluated through digital simulations and theoretical computations.
语种	中文
公开日期	2013-09-16
页码	237
内容类型	学位论文
源URL	[http://ir.ipe.ac.cn/handle/122111/1348]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	曾文炉. 微藻细胞培养与采收及其耦合研究[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2002.

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