Simultaneous determination of loading capacity and selectivity in preparative off-line two-dimensional separation: An application for purification of corilagin from Pomegranate flower extracts | |
Adili, G (Adili, Guliqire)[ 1,2 ]; Sun, GY (Sun, Guangying)[ 1 ]; Abuduaini, M (Abuduaini, Munire)[ 1,2 ]; Zhao, YX (Zhao, Yongxin)[ 1 ]; Abdulla, R (Abdulla, Rahima)[ 1 ]; Aisa, HA (Aisa, Haji Akber)[ 1,2 ] | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2020 | |
卷号 | 1622期号:7页码:1-9 |
关键词 | Capacity orthogonal chromatography Two-dimensional separation Loading capacity Orthogonality Corilagin pomegranate flower extract |
ISSN号 | 0021-9673 |
DOI | 10.1016/j.chroma.2020.461129 |
英文摘要 | This work describes the development of capacity orthogonal chromatography (COC), a new technique for simultaneously determining the loading capacity and orthogonality during the construction of two-dimensional (2D) separations. Three steps were required for the construction of a COC based on the correlation between the selectivity factor (alpha) and both orthogonality and loading capacity. (1) alpha values of the impurities-target compound were used to normalize the retention of the impurities around the target compound. (2) alpha values were input into four quadrants of a coordinate system to identify correlations between orthogonality and loading capacity. alpha values of the impurities must be greater in the first dimension than the second dimension, with iterated analyses performed until an alpha(max) is obtained for the two purification methods. (3) Touch-peak separation using the first-dimensional alpha(max) was performed and the target compound was collected. The co-eluted impurities are further separated in the second dimension To test the efficiency of this technique, a COC using two methods on a standard C18 column was developed to purify corilagin from pomegranate flower extract. Despite its low abundance, 288 mg of corilagin was obtained by COC and further purified by LH-20 gel chromatography to obtain the compound with an 80.0% recovery and 98.4% purity. Compared to COC, the purity of corilagin independently obtained using the same purification methods and identical loading capacity was poor (60.1% and 61.6%). These results indicate that COC is a useful tool for extending loading capacity in the development of preparative 2D separations. |
WOS记录号 | WOS:000536132400025 |
内容类型 | 期刊论文 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/7357] |
专题 | 新疆理化技术研究所_资源化学研究室 |
通讯作者 | Aisa, HA (Aisa, Haji Akber)[ 1,2 ] |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China |
推荐引用方式 GB/T 7714 | Adili, G ,Sun, GY ,Abuduaini, M ,et al. Simultaneous determination of loading capacity and selectivity in preparative off-line two-dimensional separation: An application for purification of corilagin from Pomegranate flower extracts[J]. JOURNAL OF CHROMATOGRAPHY A,2020,1622(7):1-9. |
APA | Adili, G ,Sun, GY ,Abuduaini, M ,Zhao, YX ,Abdulla, R ,&Aisa, HA .(2020).Simultaneous determination of loading capacity and selectivity in preparative off-line two-dimensional separation: An application for purification of corilagin from Pomegranate flower extracts.JOURNAL OF CHROMATOGRAPHY A,1622(7),1-9. |
MLA | Adili, G ,et al."Simultaneous determination of loading capacity and selectivity in preparative off-line two-dimensional separation: An application for purification of corilagin from Pomegranate flower extracts".JOURNAL OF CHROMATOGRAPHY A 1622.7(2020):1-9. |
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