Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep) | |
Nuerxiati, R (Nuerxiati, Rehebati)[ 1,3 ]; Abuduwaili, A (Abuduwaili, Aytursun)[ 1,3 ]; Mutailifu, P (Mutailifu, Paiheerding)[ 1,3 ]; Wubulikasimu, A (Wubulikasimu, Atikan)[ 1,3 ]; Rustamova, N (Rustamova, Nigora)[ 1,3 ]; Cui, JX (Cui Jingxue)[ 1,3 ]; Aisa, HA (Aisa, Haji Akber)[ 1,2 ]; Yili, A (Yili, Abulimiti)[ 1,2 ] | |
刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
2019 | |
卷号 | 141期号:12页码:431-443 |
关键词 | Orchis chusua D. Don polysaccharide Ultrasonic-assisted extraction Optimization Characterization Biological activity |
DOI | 10.1016/j.ijbiomac.2019.08.112 |
英文摘要 | In the present study, ultrasonic-assisted extraction (UAE) of polysaccharides from Orchis chusua D. Don (salep) was optimized by response surface methodology (RSM) combined with a Box-Behnken design (BBD), the optimum conditions were determined to be: ultrasonic power of 390 W, extraction temperature of 60 degrees C, extraction time of 50 min and water/raw material ratio of 40 mL/g. Based on it, 4830 +/- 0.6% yield of crude polysaccharide was obtained. After purification, the chemical compositions, physicochemical and structural property was investigated based on the GC-MS, TGA, XRD, SEM and Congo red test. The results showed that USP-U and USP-N were hetero-polysaccharides, mainly composed of mannose and glucose, and the average molecular weight was 25.57 kDa and 11.64 kDa, respectively. FT-IR spectra and NMR results indicated that the presence of a-linkage glycopyranose via typical peaks. Furthermore, reducing power and antioxidant activities of both purified fractions showed a relatively high ability to scavenge free radicals. Lastly, the cytotoxicity assay showed that purified polysaccharides have no obvious cytotoxicity against RAW267.4 and L6 cells at their effective concentration and had potent anti-inflammatory activity in a concentration-dependent manner. Salep polysaccharide is expected to be developed as a new efficacy factor in the pharmaceutical and food industry. |
WOS记录号 | WOS:000506713000046 |
内容类型 | 期刊论文 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/7280] |
专题 | 新疆理化技术研究所_资源化学研究室 |
通讯作者 | Yili, A (Yili, Abulimiti)[ 1,2 ] |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China 3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plants Resources & Chem Arid Zone, Urumqi 830011, Peoples R China |
推荐引用方式 GB/T 7714 | Nuerxiati, R ,Abuduwaili, A ,Mutailifu, P ,et al. Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,141(12):431-443. |
APA | Nuerxiati, R .,Abuduwaili, A .,Mutailifu, P .,Wubulikasimu, A .,Rustamova, N .,...&Yili, A .(2019).Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep).INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,141(12),431-443. |
MLA | Nuerxiati, R ,et al."Optimization of ultrasonic-assisted extraction, characterization and biological activities of polysaccharides from Orchis chusua D. Don (Salep)".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 141.12(2019):431-443. |
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